Implanted microvessels progress through distinct neovascularization phenotypes.
Implanted microvessels progress through distinct neovascularization phenotypes.
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DOI：
10.1016/j.mvr.2009.10.001
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发表时间：
2010-01
影响因子：
3.1
通讯作者：
Hoying JB
中科院分区：
文献类型：
--
作者：
Nunes SS;Greer KA;Stiening CM;Chen HY;Kidd KR;Schwartz MA;Sullivan CJ;Rekapally H;Hoying JB
我们之前已经证明，植入的微血管形成了一个新的微循环与最小的宿主来源的血管投资。我们的目的是确定这些植入物新生血管形成过程中的血管表型，并确定血管生成后的事件。形态学、功能和转录评估确定了植入物中三种不同的血管表型：发芽血管生成、新生血管重塑和网络成熟。首先出现的是萌芽型血管生成表型，其特点是高增殖和低壁细胞覆盖率。随后是新血管重塑表型，其特征是灌注，组织不良的新血管网络，增殖减少和重新相关的壁细胞。最后一种表型包括组织成典型树形结构的血管网络，其中包含具有正常血管周围细胞关联的血管。此外，增殖低，仅限于较大的微血管壁。从血管生成到新生血管重构的转变与植入物新生血管血流的出现一致。血管特异性和全局基因表达分析表明，中间的新血管重塑表型在转录上与其他两种表型不同。因此，这种血管表型可能不仅仅是一种过渡性表型，而是一种独特的血管表型，涉及独特的细胞和血管过程。此外，这种新生血管重构阶段可能是一般新生血管形成过程的一个正常方面。考虑到这种表型可能是功能失调的，在受损或病变组织中存在的许多微血管可能并不代表通过正常发生的新生血管表型进行适当进展的失败。
We have previously demonstrated that implanted microvessels form a new microcirculation with minimal host-derived vessel investment. Our objective was to define the vascular phenotypes present during neovascularization in these implants and identify post-angiogenesis events. Morphological, functional and transcriptional assessments identified three distinct vascular phenotypes in the implants: sprouting angiogenesis, neovascular remodeling, and network maturation. A sprouting angiogenic phenotype appeared first, characterized by high proliferation and low mural cell coverage. This was followed by a neovascular remodeling phenotype characterized by a perfused, poorly organized neovascular network, reduced proliferation, and re-associated mural cells. The last phenotype included a vascular network organized into a stereotypical tree structure containing vessels with normal perivascular cell associations. In addition, proliferation was low and was restricted to the walls of larger microvessels. The transition from angiogenesis to neovascular remodeling coincided with the appearance of blood flow in the implant neovasculature. Analysis of vascular-specific and global gene expression indicates that the intermediate, neovascular remodeling phenotype is transcriptionally distinct from the other two phenotypes. Therefore, this vascular phenotype likely is not simply a transitional phenotype but a distinct vascular phenotype involving unique cellular and vascular processes. Furthermore, this neovascular remodeling phase may be a normal aspect of the general neovascularization process. Given that this phenotype is arguably dysfunctional, many of the microvasculatures present within compromised or diseased tissues may not represent a failure to progress appropriately through a normally occurring neovascularization phenotype.
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DOI：
10.1016/s0006-291x(89)80172-x
发表时间：
1989-01-16
影响因子：
3.1
作者：
BHAGYALAKSHMI, A;FRANGOS, JA
通讯作者：
FRANGOS, JA
影响因子：
20.1
作者：
Kuehbacher, Angelika;Urbich, Carmen;Dimmeler, Stefanie
通讯作者：
Dimmeler, Stefanie
DOI：
10.1073/pnas.0705505104
发表时间：
2007-07-31
影响因子：
11.1
作者：
Abdollahi, Amir;Schwager, Christian;Huber, Peter E.
通讯作者：
Huber, Peter E.
影响因子：
3.1
作者：
Hudlická, O;Brown, MD;Silgram, H
通讯作者：
Silgram, H
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y