An LED-based UV-B irradiation system for tiny organisms: System description and demonstration experiment to determine the hatchability of eggs from four Tetranychus spider mite species from Okinawa
An LED-based UV-B irradiation system for tiny organisms: System description and demonstration experiment to determine the hatchability of eggs from four Tetranychus spider mite species from Okinawa
复制标题
DOI：
10.1016/j.jinsphys.2014.01.005
复制
发表时间：
2014-03-01
影响因子：
2.2
通讯作者：
Koveos, Dimitris S.
中科院分区：
文献类型：
--
作者：
Suzuki, Takeshi;Yoshioka, Yoshio;Koveos, Dimitris S.
我们开发了一个基于计算机的系统，用于控制来自5 x 5发光二极管（LED）矩阵（100 x 100 mm）的UV-B和白色的光周期和辐照度。在该系统中，LED矩阵安装在四个辐照箱的每一个中，并由脉宽调制器控制，使得每个箱可以分别独立地发射辐照度高达1.5和4.0Wm（-2）的UV-B和白色，或这两种光类型的组合。利用该系统对四种叶螨（Tetranychus spidermite，T. urticae、神泽叶螨T. kanzawai，T. piercei和T.冲绳亚种（okinawanus））在25 ℃下在单独的UV-B照射或与白色同时照射12 h d（-1）的条件下从冲绳岛收集。虽然在UV-B辐射下没有任何种类的卵孵化，但即使在低至0.02Wm-2的辐射下，在4.0Wm-2的白色同时照射下，孵化率也提高到>90%。在0.06Wm（-2）的UV-B、T.在白色同时照射下，冲绳种的卵孵化率为15%，而其他种的卵孵化率为10%。
We developed a computer-based system for controlling the photoperiod and irradiance of UV-B and white light from a 5 x 5 light-emitting diode (LED) matrix (100 x 100 mm). In this system, the LED matrix was installed in each of four irradiation boxes and controlled by pulse-width modulators so that each box can independently emit UV-B and white light at irradiances of up to 1.5 and 4.0 W m(-2), respectively, or a combination of both light types. We used this system to examine the hatchabilities of the eggs of four Tetranychus spider mite species (T. urticae, T. kanzawai, T. piercei and T. okinawanus) collected from Okinawa Island under UV-B irradiation alone or simultaneous irradiation with white light for 12 h d(-1) at 25 degrees C. Although no eggs of any species hatched under the UV-B irradiation, even when the irradiance was as low as 0.02 W m(-2), the hatchabilities increased to >90% under simultaneous irradiation with 4.0 W m(-2) white light. At 0.06 W m(-2) UV-B, T. okinawanus eggs hatched (15% hatchability) under simultaneous irradiation with white light, whereas other species showed hatchabilities