CONSTITUTIVE OVEREXPRESSION OF THE CYTOCHROME-P450 GENE CYP6A1 IN A HOUSE-FLY STRAIN WITH METABOLIC RESISTANCE TO INSECTICIDES
CONSTITUTIVE OVEREXPRESSION OF THE CYTOCHROME-P450 GENE CYP6A1 IN A HOUSE-FLY STRAIN WITH METABOLIC RESISTANCE TO INSECTICIDES
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DOI：
10.1016/0965-1748(94)90034-5
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发表时间：
1994-04-01
影响因子：
3.8
通讯作者：
FEYEREISEN, R
中科院分区：
文献类型：
--
作者：
CARINO, FA;KOENER, JF;FEYEREISEN, R
用克隆的cDNA探针检测了家蝇抗药性品系Diazinon-R‘Rutgers’和敏感品系Sbo细胞色素P450基因CYP6A1的信使RNA水平。在罗格斯品系中，细胞色素P6A1基因的组成性表达至少是sbo品系的10倍。在这两个品系中，用苯巴比妥处理果蝇均可诱导细胞色素P6A1的表达。对这两个菌株的基因组DNA分析表明，在罗格斯菌株中没有发现CYP6A1基因的扩增。发育分析表明，CYP6A1在幼虫和成虫中都有表达，在卵和蛹中检测到极低水平的CYP6A1 mRNA。在罗格斯品系中，无论是在幼虫还是成虫中都观察到了CYP6A1的结构性过表达。罗格斯品系与多重标记的sbo品系的杂交表明，在罗格斯品系中，CYP6A1的高结构性表达是由位于染色体II上的一个或多个基因座控制的。
Messenger RNA levels of the cytochrome P450 gene CYP6A1 were measured in the insecticide resistant Diazinon-R 'Rutgers' strain and in the susceptible strain sbo of the house fly with a cloned cDNA probe. The constitutive expression of the CYP6A1 gene was at least 10 times higher in the Rutgers strain than in the sbo strain. In both strains, CYP6A1 was inducible by phenobarbital treatment of the flies. Analysis of genomic DNA from the two strains indicated that there was no amplification of the CYP6A1 gene in the Rutgers strain. A developmental analysis revealed that CYP6A1 is expressed larvae and adults, and very low levels of CYP6A1 mRNA were detected in eggs and pupae. The constitutive overexpression of CYP6A1 in the Rutgers strain was observed in both larvae and adults. Crosses between the Rutgers strain and the multiply marked sbo strain revealed that the high constitutive expression of CYP6A1 in the Rutgers strain is controlled by one or more loci located on chromosome II.