Genetic Effects of LPIN1 Polymorphisms on Milk Production Traits in Dairy Cattle
Genetic Effects of LPIN1 Polymorphisms on Milk Production Traits in Dairy Cattle
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LPIN1 多态性对奶牛产奶性状的遗传效应
DOI：
10.3390/genes10040265
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发表时间：
2019-04-01
期刊：
影响因子：
3.5
通讯作者：
Sun, Dongxiao
中科院分区：
文献类型：
--
作者：
Han, Bo;Yuan, Yuwei;Sun, Dongxiao
本研究通过初步的RNA测序工作发现，脂蛋白1（lipin 1，LPIN 1）在奶牛干奶期、泌乳早期和泌乳高峰期差异表达，并被丰富到脂肪代谢基因本体（GO）术语和途径中，因此我们认为LPIN 1是奶牛产奶性状的候选基因。本研究在中国荷斯坦奶牛群体中检测了LPIN 1基因的多态性，并验证了其对产奶量和乳成分的遗传效应。通过对LPIN 1基因编码区和部分侧翼区序列的重测序，共发现7个SNP位点，其中5′侧翼区1个，外显子4个，3′侧翼区2个。其中，外显子中的c.637T > C、c.708A > G、c.1521C > T和c.1555A > C四个SNP被预测为导致氨基酸替换。利用Haploview4.2软件，我们发现LPIN 1的7个SNPs形成了两个单倍型区块（D′ = 0.98-1.00）。单SNP关联分析显示，SNP与第一次或第二次泌乳中的产奶量、脂肪产量、脂肪百分比或蛋白质产量显著相关（p = < 0.0001-0.0457），并且仅g.86049389C > T与两次泌乳中的蛋白质百分比强相关（p = 0.0144和0.0237）。基于单倍型的关联分析表明，两个单倍型块与产奶量、脂肪产量、蛋白质产量或蛋白质百分比显著相关（p = < 0.0001-0.0383）。实时荧光定量PCR（qRT-PCR）检测发现LPIN 1在乳腺和肝脏组织中有较高的表达。此外，我们使用SOPMA软件预测了三个SNP，c.637T > C，c.708A > G和c.1521C > T，改变了LPIN 1蛋白结构，可能是潜在的功能突变。综上所述，我们证明了LPIN 1对奶牛产奶性状的显著遗传效应，所鉴定的SNPs可作为奶牛育种的遗传标记。
Our initial RNA sequencing work identified that lipin 1 (LPIN1) was differentially expressed during dry period, early lactation, and peak of lactation in dairy cows, and it was enriched into the fat metabolic Gene Ontology (GO) terms and pathways, thus we considered LPIN1 as the candidate gene for milk production traits. In this study, we detected the polymorphisms of LPIN1 and verified their genetic effects on milk yield and composition in a Chinese Holstein cow population. We found seven SNPs by re-sequencing the entire coding region and partial flanking region of LPIN1, including one in 5′ flanking region, four in exons, and two in 3′ flanking region. Of these, four SNPs, c.637T > C, c.708A > G, c.1521C > T, and c.1555A > C, in the exons were predicted to result in the amino acid replacements. With the Haploview 4.2, we found that seven SNPs in LPIN1 formed two haplotype blocks (D′ = 0.98–1.00). Single-SNP association analyses showed that SNPs were significantly associated with milk yield, fat yield, fat percentage, or protein yield in the first or second lactation (p = < 0.0001–0.0457), and only g.86049389C > T was strongly associated with protein percentage in both lactations (p = 0.0144 and 0.0237). The haplotype-based association analyses showed that the two haplotype blocks were significantly associated with milk yield, fat yield, protein yield, or protein percentage (p = < 0.0001–0.0383). By quantitative real-time PCR (qRT-PCR), we found that LPIN1 had relatively high expression in mammary gland and liver tissues. Furthermore, we predicted three SNPs, c.637T > C, c.708A > G, and c.1521C > T, using SOPMA software, changing the LPIN1 protein structure that might be potential functional mutations. In summary, we demonstrated the significant genetic effects of LPIN1 on milk production traits, and the identified SNPs could serve as genetic markers for dairy breeding.