Development and characterization of a mouse model of duodenogastric reflux
Development and characterization of a mouse model of duodenogastric reflux
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十二指肠胃反流小鼠模型的开发和表征
DOI：
10.1016/j.lfs.2020.118412
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发表时间：
2020
期刊：
影响因子：
6.1
通讯作者：
Tian Yantao
中科院分区：
文献类型：
--
作者：
Ma Fuhai;Ma Yiming;Cui Liang;Zhao Xinhua;Wang Bingzhi;Xue Liyan;Wang Hongying;Tian Yantao
大鼠胃反流模型已被用于研究残胃癌（GSC），但其潜在的分子机制知之甚少。与大鼠不同，小鼠可以进行基因修饰，为研究与胃反流相关的GSC发育的分子机制提供了上级模型。方法将C57 BL/6小鼠随机分为对照组（n= 6）、假手术组（n= 9）和胃空肠吻合组（n= 12）。在手术后1、3和6个月处死小鼠。胃组织用苏木精和伊红染色。病变被归类为慢性炎症，肠上皮化生，或不典型增生。Key findingsNine小鼠进行胃空肠吻合术，没有死亡。胃空肠吻合组动物在术后1、3和6个月出现慢性炎症，3个月时出现肠化生（n= 2）和不典型增生（n= 1），6个月时出现肠化生（n = 2）和不典型增生（n = 2）。对照组小鼠未出现慢性炎症和肠上皮化生，而假手术组小鼠在术后1、3和6个月出现慢性炎症，未出现肠上皮化生和不典型增生。胃空肠吻合组肠化生或不典型增生比假手术组更常见（p= 0.012）。意义胃空肠吻合术可以在不进行胃切除的情况下建立十二指肠胃反流小鼠模型。
AimsRat models of duodenogastric reflux have been used to study gastric stump cancer (GSC), but the underlying molecular mechanisms are poorly understood. Unlike rats, mice can be genetically modified, providing a superior model for studying the molecular mechanisms underlying GSC development, which is associated with duodenogastric reflux. This study aimed at developing a mouse model of duodenogastric reflux.Main methodC57BL/6 mice were randomly assigned to the control (n= 6), sham operation (n= 9), or gastrojejunostomy group (n= 12). Mice were sacrificed at 1, 3, and 6 months after surgery. Stomach tissue was stained with hematoxylin and eosin. Lesions were classified as chronic inflammation, intestinal metaplasia, or atypical hyperplasia.Key findingsNine mice underwent gastrojejunostomy without mortality. The animals in the gastrojejunostomy group exhibited chronic inflammation at 1, 3, and 6 months after surgery, showing intestinal metaplasia (n= 2) and atypical hyperplasia (n= 1) at 3 months and intestinal metaplasia (n = 2) and atypical hyperplasia (n = 2) at 6 months. The mice in the control group did not exhibit chronic inflammation or intestinal metaplasia, whereas those in the sham operation group exhibited chronic inflammation at 1, 3, and 6 months after surgery, without intestinal metaplasia or atypical hyperplasia. Intestinal metaplasia or atypical hyperplasia were more common in the gastrojejunostomy group than in the sham operation group (p= 0.012).SignificanceA duodenogastric reflux mouse model can be created using gastrojejunostomy without gastrectomy.