JAGGED - A MAMMALIAN LIGAND THAT ACTIVATES NOTCH1
JAGGED - A MAMMALIAN LIGAND THAT ACTIVATES NOTCH1
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DOI：
10.1016/0092-8674(95)90294-5
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发表时间：
1995-03-24
期刊：
影响因子：
64.5
通讯作者：
WEINMASTER, G
中科院分区：
文献类型：
--
作者：
LINDSELL, CE;SHAWBER, CJ;WEINMASTER, G
在这里，我们报告分离的大鼠cDNA克隆，锯齿状，我们显示编码脊椎动物Notch的配体。我们的结论是基于三个观察。首先，序列分析揭示了LIN-12/Notch蛋白的Jagged和无脊椎动物配体之间的实质性同源性。第二，大鼠胚胎的原位杂交鉴定了Jagged与Notch 1，Notch 2和Notch 3的基因表达的不同和重叠模式。最后，使用细胞培养测定测试Jagged的生物活性，其中Jagged激活成肌细胞中表达的大鼠Notch 1并阻止肌肉细胞分化。我们的数据支持Notch-配体相互作用在维持哺乳动物细胞处于未分化状态中起作用的假设。
Here we report the isolation of a rat cDNA clone, Jagged, which we show encodes a ligand for vertebrate Notch. Our conclusion is based on three observations. First, sequence analysis reveals substantial homology between Jagged and invertebrate ligands for the LIN-12/Notch proteins. Second, in situ hybridization of rat embryos identifies both distinct and overlapping patterns of gene expression for Jagged with those for Notch1, Notch2, and Notch3. Finally, the biological activity of Jagged was tested using a cell culture assay in which Jagged activates rat Notch1 expressed in myoblasts and prevents muscle cell differentiation. Our data support the hypothesis that Notch-ligand interactions function in maintaining mammalian cells in an undifferentiated state.