Two Distinct Types of Theca Cells in the Medaka Gonad: Germ Cell-Dependent Maintenance of cyp19a1-Expressing Theca Cells
Two Distinct Types of Theca Cells in the Medaka Gonad: Germ Cell-Dependent Maintenance of cyp19a1-Expressing Theca Cells
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DOI：
10.1002/dvdy.22068
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发表时间：
2009-10-01
影响因子：
2.5
通讯作者：
Tanaka, Minoru
中科院分区：
文献类型：
--
作者：
Nakamura, Shuhei;Kurokawa, Hiromi;Tanaka, Minoru
芳香酶是一种类固醇合成酶，催化雌激素的产生，对生殖系统的正常发育和功能至关重要。用绿色荧光蛋白荧光重现内源性CYP19A1表达的转基因青竹(Oryzias Layzias)，分析了发育中性腺中表达卵巢芳香酶的细胞的谱系。我们的结果表明，在发育中的女性性腺的腹侧基质细胞中出现了表达CYP19A1的细胞，然后随着性腺区的向前延伸而向前扩张。这些细胞位于发育中的卵泡附近，与表达P450c17-I的膜细胞不同。在成体卵巢中，P450c17-I和CYP19A1在外膜细胞层的表达是相互排斥的。在颗粒细胞中，仅在大卵泡中有表达。这些观察证明了青竹卵巢中有两种膜细胞。我们还表明，表达CYP19A1的细胞的维持依赖于生殖细胞。发展动力学238.2652-2657年，2009年。(C)2009年Wiley-Liss，Inc.
Aromatase is a steroidogenic enzyme catalyzing the production of estrogens and is important for the proper development and function of the reproductive system. The lineage of cyp19a1 (ovarian-type aromatase)expressing cells in the developing gonad was analyzed using a transgenic medaka (Oryzias latipes) that recapitulates endogenous cyp19a1 expression with EGFP fluorescence. Our results show that cyp19a1-expressing cells arise in the ventral stromal cells of the developing female gonad, then expand anteriorly as the gonadal region extends anteriorly. These cells become located close to the developing follicles, and are distinguishable from the P450c17-I-expressing theca cells. In the adult ovary, the expression of P450c17-I and cyp19a1 are mutually exclusive in the outer theca-cell layer. Cyp19a1 expression in the granulosa cells is found only in the population of large follicles. These observations demonstrate two types of theca cells in the medaka ovary. We also show that the maintenance of cyp19a1-expressing cells depends on germ cells. Developmental Dynamics 238.2652-2657, 2009. (c) 2009 Wiley-Liss, Inc.