Fabrication of High-Density Vertical Closed Bipolar Electrode Arrays by Carbon Paste Filling Method for Two-Dimensional Chemical Imaging
Fabrication of High-Density Vertical Closed Bipolar Electrode Arrays by Carbon Paste Filling Method for Two-Dimensional Chemical Imaging
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DOI：
10.1021/acs.analchem.1c05354
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发表时间：
2022-06-14
影响因子：
7.4
通讯作者：
Shiku, Hitoshi
中科院分区：
文献类型：
--
作者：
Iwama, Tomoki;Inoue, Kumi Y.;Shiku, Hitoshi
在这项研究中，提出了一种碳糊填充方法作为制造用于双极电化学显微镜（BEM）的高密度双极电极（BPE）阵列的简单策略。使用制造的 BPE 阵列实现了高时空分辨率成像。 BEM是近年来新兴的显微系统，利用高密度BPE阵列和电化学发光（ECL）信号实现分子分布的无标记、高时空分辨率成像。我们设计了一种简单的方法来制造 BPE 阵列，即在多孔板中填充碳糊，并成功制造出高密度 BPE 阵列（15 μm 间距）。使用扫描电子显微镜详细观察BPE阵列的表面后，使用铁氰化钾溶液作为样品溶液评估基本电化学和ECL发射特性。此外，对样品分子进行流入成像以评估所制备的BPE阵列的成像能力。此外，将含有普鲁士蓝的碳墨水应用于BPE阵列的样品溶液侧，为过氧化氢提供催化活性，并实现了ECL信号对过氧化氢的定量和流入成像。这种简单的 BPE 阵列制造方法可以加速 BEM 的研究和开发。此外，边界元法过氧化氢成像是实现高时空分辨率生物成像（例如使用酶的生物分子成像）的一个重要里程碑。
In this study, a carbon paste filling method was proposed as a simple strategy for fabricating high-density bipolar electrode (BPE) arrays for bipolar electrochemical microscopy (BEM). High spatiotemporal resolution imaging was achieved using the fabricated BPE array. BEM, which is an emerging microscopic system in recent years, achieves label-free and high spatiotemporal resolution imaging of molecular distributions using high-density BPE arrays and electrochemiluminescence (ECL) signals. We devised a simple method to fabricate a BPE array by filling a porous plate with carbon paste and succeeded in fabricating a high-density BPE array (15 mu m pitch). After a detailed observation of the surface of the BPE array using a scanning electron microscope, the basic electrochemical and ECL emission characteristics were evaluated using potassium ferricyanide solution as a sample solution. Moreover, inflow imaging of the sample molecules was conducted to evaluate the imaging ability of the prepared BPE array. In addition, Prussian Blue containing carbon ink was applied to the sample solution side of the BPE array to provide catalytic activity to hydrogen peroxide, and the quantification and inflow imaging of hydrogen peroxide by ECL signals was achieved. This simple fabrication method of the BPE array can accelerate the research and development of BEM. Furthermore, hydrogen peroxide imaging by BEM is an important milestone for achieving bioimaging with high spatiotemporal resolution such as biomolecule imaging using enzymes.