NUP82 is an essential yeast nucleoporin required for poly(A)+ RNA export.
NUP82 is an essential yeast nucleoporin required for poly(A)+ RNA export.
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DOI：
10.1083/jcb.130.6.1275
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发表时间：
1995-09
期刊：
影响因子：
--
通讯作者：
Blobel G
中科院分区：
文献类型：
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作者：
Hurwitz ME;Blobel G
我们已经分离和表征了编码一种新的82 kD的必需核孔蛋白的基因，称为NUP 82。含有NUP 82的表位标记拷贝的细胞的间接免疫荧光将其定位于核孔复合物（NPC）。一级结构分析表明，COOH-末端195个氨基酸含有一个假定的卷曲螺旋结构域。该结构域的COOH末端87个氨基酸的缺失导致细胞生长较慢; COOH末端108个氨基酸的缺失导致在30 ℃下生长较慢，在37 ℃下致死。NUP 82的最后108个氨基酸已被删除的细胞，当转移到37 ℃时，在其核孔复合物或核膜中不显示任何总体形态学缺陷。然而，它们在37摄氏度时会在细胞核中积累poly（A）+ RNA。我们建议NUP 82作为一个连接器，通过其卷曲螺旋结构域将直接参与核转运的核孔蛋白连接到孔支架上。
We have isolated and characterized the gene encoding a novel essential nucleoporin of 82 kD, termed NUP82. Indirect immunofluorescence of cells containing an epitope tagged copy of the NUP82 localized it to the nuclear pore complex (NPC). Primary structure analysis indicates that the COOH-terminal 195 amino acids contain a putative coiled-coil domain. Deletion of the COOH-terminal 87 amino acids of this domain causes slower cell growth; deletion of the COOH-terminal 108 amino acids results in slower growth at 30 degrees C and lethality at 37 degrees C. Cells in which the last 108 amino acids of NUP82 have been deleted, when shifted to 37 degrees C, do not display any gross morphological defects in their nuclear pore complexes or nuclear envelopes. They do, however, accumulate poly(A)+ RNA in their nuclei at 37 degrees C. We propose that NUP82 acts as a linker to tether nucleoporins directly involved in nuclear transport to pore scaffolding via its coiled-coil domain.