ANDROGEN RECEPTOR LOCALIZATION IN DIFFERENT CELL-TYPES OF THE ADULT-RAT PROSTATE
ANDROGEN RECEPTOR LOCALIZATION IN DIFFERENT CELL-TYPES OF THE ADULT-RAT PROSTATE
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DOI：
10.1210/endo-129-6-3187
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发表时间：
1991-12-01
期刊：
影响因子：
4.8
通讯作者：
GREENE, GL
中科院分区：
文献类型：
--
作者：
PRINS, GS;BIRCH, L;GREENE, GL
为了更好地了解雄激素对大鼠前列腺生长和功能的直接和间接作用，对完整的成年前列腺腹叶、背叶和侧叶内的各种细胞群进行了雄激素受体（AR）存在或不存在的表征。 针对大鼠AR（PG-21）的氨基酸1-21产生的多克隆兔抗体与针对细胞特异性抗原的单克隆抗体库联合用于阳性细胞鉴定。 腔上皮细胞AR呈强阳性，阳性顺序为腹侧>外侧大于或等于背侧。 在外侧叶，染色强度最强的周边地区，而类似的梯度是不明显的腹侧和背侧前列腺。 基底上皮细胞AR阴性的所有区域的三个叶。 腺泡周围平滑肌强阳性AR，这种染色不随肌肉层的厚度而变化。 血管内皮细胞AR阴性，而血管周围平滑肌细胞AR阳性。 尽管在腹叶和背叶内观察到一些AR阳性成纤维细胞外观细胞，但大多数基质成纤维细胞为AR阴性。 ED 2染色，组织巨噬细胞的特异性标志物，显示固定的巨噬细胞在完整的大鼠前列腺叶的基质中存在大量。 由于这些细胞经常被鉴定为AR阳性，因此巨噬细胞可能部分解释了AR阳性基质细胞的出现。 因此，目前的研究结果表明，在三个前列腺叶的不同细胞类型之间的AR表达的复杂模式。 表达AR的细胞类型可能被认为是雄激素作用的直接靶点，而缺乏AR的细胞应被认为是间接靶点或雄激素不敏感细胞。
To better understand direct and indirect androgen action on rat prostatic growth and function, the various cell populations within the intact adult ventral, dorsal, and lateral prostate lobes were characterized for the presence or absence of androgen receptor (AR). Polyclonal rabbit antibodies raised against amino acids 1-21 of the rat AR (PG-21) were used in combination with a library of monoclonal antibodies directed against cell-specific antigens for positive cellular identification. Luminal epithelial cells were strongly AR positive, with an order of ventral > lateral greater-than-or-equal-to dorsal. In the lateral lobe, staining intensity was strongest in the peripheral regions, whereas a similar gradient was not apparent in the ventral and dorsal prostate. Basal epithelial cells were AR negative in all regions of the three lobes. Periacinar smooth muscle was strongly positive for AR, and this staining did not vary with the thickness of the muscle layer. Endothelial cells of the vasculature were AR negative, while the perivascular smooth muscle cells were AR positive. The majority of stromal fibroblasts were AR negative, although a number of AR-positive fibroblastic-appearing cells were observed within the ventral and dorsal lobes. Staining with ED2, a specific marker for tissue macrophages, revealed that fixed macrophages were present in significant quantities in the stroma of intact rat prostate lobes. Since these were frequently identified as AR positive, macrophages may partially account for the appearance of AR-positive stromal cells. Thus, the present findings indicate a complex pattern of AR expression among different cell types of the three prostate lobes. Cells types that express AR can potentially be considered as direct targets of androgen action, whereas those lacking AR should be considered as indirect targets or androgen-insensitive cells.