Efficient induction of oral tolerance by fusing cholera toxin B subunit with allergen-specific T-cell epitopes accumulated in rice seed.
Efficient induction of oral tolerance by fusing cholera toxin B subunit with allergen-specific T-cell epitopes accumulated in rice seed.
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DOI：
10.1016/j.vaccine.2008.09.019
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发表时间：
2008-11
期刊：
影响因子：
5.5
通讯作者：
H. Takagi;T. Hiroi;Lijun Yang;K. Takamura;R. Ishimitsu;H. Kawauchi;F. Takaiwa
中科院分区：
文献类型：
--
作者：
H. Takagi;T. Hiroi;Lijun Yang;K. Takamura;R. Ishimitsu;H. Kawauchi;F. Takaiwa
霍乱毒素B（CT B）亚基是一种有效的粘膜载体分子，用于诱导对抗原和过敏原的口服耐受。在这里，在日本雪松花粉中的主要过敏原的Cry j 1和Cry j 2的T细胞表位在水稻种子中表达为与CTB或水稻谷蛋白作为对照的融合蛋白。用含有CTB融合T细胞表位的水稻种子喂养小鼠，可以抑制过敏原特异性IgE反应和花粉诱导的临床症状，T细胞表位的剂量比使用对照种子时低50倍。我们的发现为I型过敏的免疫治疗提供了一种新的潜在策略。
Cholera toxin B (CTB) subunit is an efficient mucosal carrier molecule for induction of oral tolerance to antigens and allergens. Here, T-cell epitopes of Cry j 1 and Cry j 2, major allergens in Japanese cedar pollen, were expressed in rice seed as a fusion protein with either CTB or rice glutelin as a control. Feeding mice with rice seed containing CTB-fused T-cell epitopes suppressed allergen-specific IgE responses and pollen-induced clinical symptoms at 50-fold lower doses of T-cell epitopes than required when using control seed. Our findings present a novel potential strategy for immunotherapy of type-I allergy.