Staphylococcus aureus domain V functions in Escherichia coli ribosomes provided a conserved interaction with domain IV is restored.
Staphylococcus aureus domain V functions in Escherichia coli ribosomes provided a conserved interaction with domain IV is restored.
复制标题
只要恢复与结构域 IV 的保守相互作用，金黄色葡萄球菌结构域 V 就能在大肠杆菌核糖体中发挥作用。
DOI：
10.1017/s1355838201010172
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Dahlberg,AE
中科院分区：
文献类型：
--
作者：
Thompson,J;Tapprich,WE;Munger,C;Dahlberg,AE
Domain V of Escherichia coli 23 S rRNA (residues 2023-2630) was replaced by that from Staphylococcus aureus, thereby introducing 132 changes in the rRNA sequence. The resulting ribosomal mutant was unable to support cell growth. The mutant was rescued, however, by restoring an interaction between domains IV and V (residues 1782 and 2586). Although the importance of this interaction, U/U in E. coli, C/C in S. aureus, is therefore demonstrated, it cannot be the only tertiary interaction important for ribosomal function as the rescued hybrid grew more slowly than the wild type. Additionally, although the single-site mutations U1782C and U2586C in E. coli are viable, the double mutant is lethal.
