Structural changes of lignocelluloses by a nonionic surfactant, Tween 20, and their effects on cellulase adsorption and saccharification
Structural changes of lignocelluloses by a nonionic surfactant, Tween 20, and their effects on cellulase adsorption and saccharification
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DOI：
10.1016/j.biortech.2011.07.034
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发表时间：
2011-10-01
影响因子：
11.4
通讯作者：
Sakoda, Akiyoshi
中科院分区：
文献类型：
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作者：
Seo, Dong-June;Fujita, Hirotaka;Sakoda, Akiyoshi
在这项工作中，我们发现吐温20处理（0-8 mM）的细胞壁的崩溃，除了那些与高木质素含量和高结晶度的大多数样品。细胞壁坍塌导致10- 50-nm孔的形成，不仅使纤维素酶的单层饱和吸附量增加约3-3.6倍，而且使纤维素酶吸附速率（D-e/r（2））增加约160-880倍。Tween 20处理72 h后纤维素转化率提高8.7-21.5%。另一方面，Avicel（微晶纤维素）对吐温20的吸附阻碍了纤维素酶反应（吸附和糖化）。Tween 20处理对木质纤维素和Avicel的结晶部分的影响不显著。结果发现，一定程度的预处理（如木质素去除），提高吐温20扩散到样品中是必要的，以获得吐温20的结构效果。(C)2011爱思唯尔有限公司保留所有权利。
In this work, we found that Tween 20 treatment (0-8 mM) contributed to the cell wall collapse of most samples except for those with high lignin contents and high crystallinity. Cell wall collapse contributed to the formation of 10- to 50-nm pores and not only increased the monolayer saturation amount of adsorbed cellulase about 3-3.6 times but also increased the cellulase adsorption rate (D-e/r(2)) about 160-880 times. Moreover, cellulose conversion at 72 h was also increased 8.7-21.5% by Tween 20 treatment. On the other hand, the adsorption of Tween 20 on Avicel (microcrystalline cellulose) hindered the cellulase reaction (adsorption and saccharification). The effect of Tween 20 treatment on the crystalline part was insignificant for both lignocelluloses and Avicel. It was found that some degree of pretreatment (e.g. lignin removal) that enhances Tween 20 diffusion into samples is necessary to obtain the structural effects of Tween 20. (C) 2011 Elsevier Ltd. All rights reserved.