Microglial subpopulations with distinct transcriptome signatures vary across brain regions in the resting mouse brain
Microglial subpopulations with distinct transcriptome signatures vary across brain regions in the resting mouse brain
复制标题
具有不同转录组特征的小胶质细胞亚群在静息小鼠大脑中的不同大脑区域有所不同
DOI：
10.1016/j.jphs.2022.12.010
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发表时间：
2023
影响因子：
3.5
通讯作者：
Furuyashiki Tomoyuki
中科院分区：
文献类型：
--
作者：
Mishima Rei;Taniguchi Masayuki;Matsushita Kazutoshi;Tian Bowen;Furuyashiki Tomoyuki
小胶质细胞对组织内环境的稳定和紊乱至关重要。然而，小胶质细胞的异质性及其与生理条件下小胶质细胞活化的关系仍然是难以捉摸的。使用单细胞RNA测序，我们确定了小胶质细胞亚群与不同的转录组签名在休息的大脑。两个主要的，连续的亚群分布在不同的大脑区域，特别是大脑皮质和下丘脑之间。脂多糖和慢性社会失败压力，这两者都涉及先天免疫受体TLR4，上调选择性小胶质细胞亚群的标记基因。这些发现表明，小胶质细胞亚群有助于小胶质细胞转录组的异质性和反应性内和跨脑区域。
Microglia are crucial for tissue homeostasis and its disturbance. However, microglial heterogeneity and its relationship with microglial activation in physiological conditions remain elusive. Using single-cell RNA sequencing, we identified microglial subpopulations with distinct transcriptome signatures in the resting brain. The distribution of two major, continuous subpopulations varied across brain regions, especially between cerebral cortices and the hypothalamus. Lipopolysaccharide and chronic social defeat stress, both of which involve the innate immune receptor TLR4, upregulate the marker genes of selective microglial subpopulations. These findings suggest that microglial subpopulations contribute to the heterogeneity of microglial transcriptome and responsiveness within and across brain regions.