Phagocytosis of dying chondrocytes by osteoclasts in the mouse growth plate as demonstrated by annexin-V labelling
Phagocytosis of dying chondrocytes by osteoclasts in the mouse growth plate as demonstrated by annexin-V labelling
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DOI：
10.1007/s004410000238
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发表时间：
2000-08-01
影响因子：
3.6
通讯作者：
van den Eijnde, SM
中科院分区：
文献类型：
--
作者：
Bronckers, ALJJ;Goei, W;van den Eijnde, SM
长骨骨骺生长板中的软骨内骨化与大部分软骨细胞的程序性细胞死亡（PCD）有关。我们是否测试了以下假设：在骨骺生长板的骨化前沿，破骨细胞优先吞噬正在进行 PCD 的软骨细胞。我们向年轻成年小鼠静脉注射生物素标记的膜联蛋白-V（anx-V-生物素，PCD 的早期标记物）。标记30分钟后，回收长骨，并使用ABC-过氧化物酶组织化学检查生长板中anx-V-生物素标记细胞的组织分布，在距离骨化前沿一定距离的封闭腔隙中仍然存在的肥大软骨细胞中检测到anx-V-生物素阳性染色。在骨化前沿，软骨细胞陷窝打开，耐酒石酸酸性磷酸酶阳性破骨细胞与肥大软骨细胞紧密接触。破骨细胞与 anx-V-生物素标记的软骨细胞接触的频率明显高于与未标记软骨细胞的接触。破骨细胞的细胞质内还含有标记和未标记的吞噬细胞片段。我们得出的结论是，在生长板中，破骨细胞优先吞噬正在死亡的肥大软骨细胞，这表明这些死亡细胞可能向破骨细胞发出信号，要求其去除。
Endochondral ossification in the epiphyseal growth plate of long bones is associated with programmed cell death (PCD) of a major portion of the chondrocytes. Were we tested the hypothesis that at the ossification front of the epiphyseal growth plate osteoclasts preferentially phagocytose chondrocytes that are undergoing PCD. We injected biotin-labelled annexin-V (anx-V-biotin, an early marker of PCD) intravenously in young adult mice. After 30 min of labelling, long bones were recovered and the tissue distribution examined of anx-V-biotin-labelled cells in the growth plate using ABC-peroxidase histochemistry, Positive staining for anx-V-biotin was detected in hypertrophic chondrocytes still present in closed lacunae at some distance from the ossification front. At the ossification front, chondrocyte lacunae were opened and close contacts were seen between tartrate-resistant acid phosphatase-positive osteoclasts and hypertrophic cartilage cells. Osteoclasts were significantly moro frequently in contact with anx-V-biotin-labelled chondrocytes than with unlabelled chondrocytes. Osteoclasts also contained labelled and unlabelled phagocytic fragments within their cytoplasm. We conclude that in the growth plate osteoclasts preferentially phagocytose hypertrophic chondrocytes that are dying, suggesting these dying cells may signal osteoclasts For their removal.