Reversible Thermoresponsive Peptide-PNIPAM Hydrogels for Controlled Drug Delivery
Reversible Thermoresponsive Peptide-PNIPAM Hydrogels for Controlled Drug Delivery
复制标题
用于受控药物输送的可逆温敏肽-PNIPAM水凝胶
DOI：
10.1021/acs.biomac.9b01009
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发表时间：
2019-09-01
影响因子：
6.2
通讯作者：
Lu, Jian Ren
中科院分区：
文献类型：
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作者：
Cao, Meiwen;Wang, Yu;Lu, Jian Ren
混合热可逆凝胶成功地制造了通过添加热敏聚合物，聚（N-异丙基丙烯酰胺）（PNIPAM），纤维状纳米结构自组装从短肽I3 K。当温度升高到PNIPAM的较低临界溶解温度以上时，分子塌陷形成凝聚的球状颗粒，其充当交联剂以连接不同的肽纳米原纤维并冻结它们的运动，导致水凝胶的形成。由于这些过程是物理驱动的，这样的水凝胶可以作为温度的函数在溶胶和凝胶状态之间可逆地切换。作为模型肽，I3 K与PNIPAM一起配制以产生具有类似于33摄氏度的转变温度的热可逆溶胶-凝胶系统，该转变温度刚好低于体温。抗菌肽G（IIKK）（3）I-NH 2可以通过在较低温度下在溶胶相中加入溶液，然后将温度升高到33 ℃以上进行凝胶化而方便地包封在水凝胶中。该水凝胶对G（IIKK）（3）I-NH 2的释放呈线性、持续释放。使用肽纳米原纤维作为三维支架，这种温敏水凝胶模拟细胞外基质，并可能用作微创药物递送或组织工程的可注射水凝胶。
Mixed thermoreversible gels were successfully fabricated by the addition of a thermosensitive polymer, poly(N-isopropylacrylamide) (PNIPAM), to fibrillar nanostructures self-assembled from a short peptide I3K. When the temperature was increased above the lower critical solution temperature of the PNIPAM, the molecules collapsed to form condensed globular particles, which acted as cross-links to connect different peptide nanofibrils and freeze their movements, resulting in the formation of a hydrogel. Since these processes were physically driven, such hydrogels could be reversibly switched between the sol and gel states as a function of temperature. As a model peptide, I3K was formulated with PNIPAM to produce a thermoreversible sol-gel system with a transition temperature of similar to 33 degrees C, which is just below the body temperature. The antibacterial peptide of G(IIKK)(3)I-NH2 could be conveniently encapsulated in the hydrogel by the addition of the solution at lower temperatures in the sol phase and then increasing the temperature to be above 33 degrees C for gelation. The hydrogel gave a sustained and controlled linear release of G(IIKK)(3)I-NH2 over time. Using the peptide nanofibrils as three-dimensional scaffolds, such thermoresponsive hydrogels mimic the extracellular matrix and could potentially be used as injectable hydrogels for minimally invasive drug delivery or tissue engineering.