STRUCTURE OF MEMBRANE DOMAINS AND MATRIX COMPONENTS OF THE BOVINE ACROSOME
STRUCTURE OF MEMBRANE DOMAINS AND MATRIX COMPONENTS OF THE BOVINE ACROSOME
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DOI：
10.1016/0889-1605(85)90113-2
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
WINFREY, VP
中科院分区：
文献类型：
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作者：
OLSON, GE;WINFREY, VP
采用薄切片、冷冻断裂、表面复型和负染技术对牛精子顶体膜系统进行了研究，以确定特定顶体膜结构域的结构分化。顶体和主段的外顶体膜的特征在于与其腔面和随机的膜内颗粒分布相关的突出的电子致密复合物。在赤道段，2维组织之间的外顶体膜和内顶体膜延伸的桥接元件被确定和相关的冷冻断裂图像。内顶体膜缺乏外顶体膜上观察到的电子致密组装，并且在冷冻断裂中它呈现结晶状。进一步的研究确定了电子致密的顶体下物质分布在顶体内膜和外核膜之间的空间中。提出了关于顶体基质结构组织的新观察结果。
The acrosomal membrane system of bovine spermatozoa was examined by thin-section, freeze-fracture, surface-replica and negative staining techniques to identify structural differentiations of specific acrosomal membrane domains. The outer acrosomal membrane of the apical and principal segments is characterized by a prominent electron-dense complex associated with its luminal face and a random intramembraneous particle distribution. In the equatorial segment, the 2-dimensional organization of bridging elements extending between the outer and inner acrosomal membrane was determined and correlated to freeze-fracture images. The inner acrosomal membrane lacked the electron-dense assembly noted on the outer acrosomal membrane and in freeze-fracture it appears crystalline. further studies identified the distribution of the electron-dense subacrosomal material in the space between the inner acrosomal membrane and outer nuclear membrane. New observations on the structural organization of the acrosomal matrix are presented.