Combined effects of protein synthesis and phosphorylation inhibitors on maturation of mouse oocytes in vitro
Combined effects of protein synthesis and phosphorylation inhibitors on maturation of mouse oocytes in vitro
复制标题
蛋白质合成和磷酸化抑制剂对小鼠卵母细胞体外成熟的联合影响
DOI：
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发表时间：
1990
影响因子：
2.5
通讯作者：
Z. Rimkevičová
中科院分区：
文献类型：
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作者：
J. Motlík;Z. Rimkevičová
在裸露的小鼠卵母细胞中，在dbcAMP（1 mM）和放线菌酮（10 μg/ml）中预孵育3小时或5小时，然后仅在放线菌酮中再孵育3小时，都不能降低GVBD率（分别为93%和92%）。这意味着在放线菌酮中预孵育3和5小时不会损害小鼠卵母细胞在含有蛋白质合成抑制剂的培养基中恢复减数分裂的能力。为了测试抑制蛋白磷酸化和蛋白合成的组合效应，将卵母细胞在2 mM 6-DMAP中培养3、4或5小时，随后在10 μg/ml放线菌酮中培养3小时。当随后的蛋白质合成被放线菌酮阻断时，在6-DMAP中孵育4或5小时降低了小鼠卵母细胞恢复减数分裂的能力（分别为GVBD的63%或35%）。然而，高度浓缩的二价体总是可见的GV。因此，尽管GVBD被阻断，但上述处理不能防止染色质凝聚。
In denuded mouse oocytes, neither 3 nor 5 hours of preincubation in dbcAMP (1 mM) and cycloheximide (10 μg/ml), followed by further 3 hours in cycloheximide only, lowered the rate of GVBD (93% and 92%, respectively). It means that 3 and 5 hours preincubation in cycloheximide did not impair the ability of mouse oocytes to resume meiosis in medium with the protein synthesis inhibitor. To test the combined effects of inhibition of protein phosphorylation and protein synthesis, oocytes were cultured for 3, 4, or 5 hours in 2 mM of 6‐DMAP and subsequently for 3 hours in 10 μg/ml cycloheximide. The incubation in 6‐DMAP for 4 or 5 hours diminished (63% or 35% of GVBD, respectively) the ability of mouse oocytes to resume meiosis when subsequent protein synthesis was blocked by cycloheximide. However, the highly condensed bivalents were always visible in GVs. Thus the above treatment did not prevent chromatin condensation although GVBD was blocked.