ANTINEUTROPHIL ANTIBODIES IN INFLAMMATORY BOWEL-DISEASE - PREVALENCE AND DIAGNOSTIC ROLE
ANTINEUTROPHIL ANTIBODIES IN INFLAMMATORY BOWEL-DISEASE - PREVALENCE AND DIAGNOSTIC ROLE
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DOI：
10.1136/gut.33.5.668
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发表时间：
1992-05-01
期刊：
影响因子：
24.5
通讯作者：
LEAKER, B
中科院分区：
文献类型：
--
作者：
CAMBRIDGE, G;RAMPTON, DS;LEAKER, B
在各种炎症性疾病患者的血清中都显示出抗中性粒细胞抗体。那些与中性粒细胞胞浆成分发生反应的细胞与系统性血管炎有关。使用炎症性肠病患者的血清，已经报道了人中性粒细胞的核和核周染色模式。我们评估了100名炎症性肠病患者、14名疾病对照组和20名正常志愿者的血清对人中性粒细胞的反应性。溃疡性结肠炎患者血清中有27/50(54%)的血清含有与胞浆乙醇固定的中性粒细胞反应的抗体，而克罗恩病患者(P<；0.001)和对照血清(P<；0.001)的抗体含量分别为5/50(10%)和0/34(p&t；0.001)。所有7例单纯直肠炎患者的血清均为阴性(p<；0.01)。抗中性粒细胞抗体的存在或滴度与疾病活动性或治疗无关。阳性血清对人中性粒细胞有三种不同的染色模式。以核周为主(17/32)，12份血清呈胞浆染色，3份血清呈均一核染色。患者或对照组均无髓过氧化物酶、弹性蛋白酶或丝氨酸蛋白酶3抗体，所有这些抗体均可被抗中性粒细胞胞浆抗体识别。27份间接免疫荧光阳性血清中，仅2份与中性粒细胞初级颗粒提取物反应。总而言之，抗中性粒细胞抗体在溃疡性结肠炎中比克罗恩病或对照组中更常见，在炎症性肠病中发现的抗中性粒细胞抗体与与血管炎有关的抗体是不同的。
Anti-neutrophil antibodies have been shown in sera from patients with a variety of inflammatory diseases. Those reacting with components of neutrophil cytoplasm are associated with systemic vasculitis. Both nuclear and perinuclear staining patterns on human neutrophils have been reported using sera from patients with inflammatory bowel disease. We have evaluated the reactivity against human neutrophils of sera from 100 patients with inflammatory bowel disease, 14 disease controls, and 20 normal volunteers. Altogether 27/50 (54%) sera from patients with ulcerative colitis contained antibodies that reacted with cytospun ethanol fixed neutrophils compared with 5/50 (10%) from Crohn's disease (p < 0.001) and 0/34 control sera (p < 0.001). All seven sera from patients with proctitis alone were negative (p < 0.01). There was no correlation between presence or titre of anti-neutrophil antibodies and either disease activity or treatment. Positive sera gave three different staining patterns on human neutrophils. The predominant pattern was peri-nuclear (17/32); 12 sera gave a cytoplasmic and three a homogeneous nuclear staining pattern. None of the patients or the controls had antibodies to myeloperoxidase, elastase, or serine proteinase 3, all of which are recognised by anti-neutrophil cytoplasmic antibodies. Only 2/27 sera positive by indirect immunofluorescence reacted with an extract of neutrophil primary granules. In conclusion, anti-neutrophil antibodies occur more commonly in ulcerative colitis than in Crohn's disease or control subjects and the anti-neutrophil antibodies found in inflammatory bowel disease are different from those associated with Vasculitis.