Cardiac norepinephrine transporter protein expression is inversely correlated to chamber norepinephrine content.
Cardiac norepinephrine transporter protein expression is inversely correlated to chamber norepinephrine content.
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DOI：
10.1152/ajpregu.00190.2008
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发表时间：
2008-09
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影响因子：
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通讯作者：
E. Wehrwein;L. M. Parker;A. Wright;J. Spitsbergen;M. Novotný;D. Babánková;G. Swain;B. Habecker;D. Kreulen
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文献类型：
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作者：
E. Wehrwein;L. M. Parker;A. Wright;J. Spitsbergen;M. Novotný;D. Babánková;G. Swain;B. Habecker;D. Kreulen
交感神经元中的心脏神经元去甲肾上腺素转运体（NET）负责从神经效应器接点摄取释放的去甲肾上腺素。本研究的目的是评估使用[(3)H]尼西汀结合大鼠心膜测量的心脏净网蛋白的腔室分布，并将NE含量与净网量联系起来。在整个心房中，NET共定位于神经纤维中，具有酪氨酸羟化酶（TH）免疫反应性。以微克/克组织表示的NE含量在心室最低；而左心室的NET结合明显高于右心室和心房（P < 0.05），呈显著负相关(r(2) = 0.922；P < 0.05)。神经毒素6-羟多巴胺（一种NET底物）在心室比心房更能降低NE含量，这表明高心室NET结合的功能意义。综上所述，脑室网结合的优势与脑室高的NE再摄取能力相对应，但与组织NE含量呈负相关。
The cardiac neuronal norepinephrine (NE) transporter (NET) in sympathetic neurons is responsible for uptake of released NE from the neuroeffector junction. The purpose of this study was to assess the chamber distribution of cardiac NET protein measured using [(3)H]nisoxetine binding in rat heart membranes and to correlate NE content to NET amount. In whole mounts of atria, NET was colocalized in nerve fibers with tyrosine hydroxylase (TH) immunoreactivity. NE content expressed as micrograms NE per gram tissue was lowest in the ventricles; however, NET binding was significantly higher in the left ventricle than the right ventricle and atria (P < 0.05), resulting in a significant negative correlation (r(2) = 0.922; P < 0.05) of NET to NE content. The neurotoxin 6-hydroxydopamine, an NET substrate, reduced NE content more in the ventricles than the atria, demonstrating functional significance of high ventricular NET binding. In summary, there is a ventricular predominance of NET binding that corresponds to a high NE reuptake capacity in the ventricles, yet negatively correlates to tissue NE content.