Morphological, Photosynthetic, and Physiological Responses of Rapeseed Leaf to Different Combinations of Red and Blue Lights at the Rosette Stage.
Morphological, Photosynthetic, and Physiological Responses of Rapeseed Leaf to Different Combinations of Red and Blue Lights at the Rosette Stage.
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DOI：
10.3389/fpls.2016.01144
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发表时间：
2016
影响因子：
5.6
通讯作者：
Rongzhan G
中科院分区：
文献类型：
--
作者：
Shengxin C;Chunxia L;Xuyang Y;Song C;Xuelei J;Xiaoying L;Zhigang X;Rongzhan G
油菜（Brassica napus L.）对光线质量很敏感。工厂化生产蔬菜用和大田移栽用油菜苗需要研究油菜对光质的反应。本研究以油菜为试材，研究了油菜叶片对不同浓度外源激素的反应。“中双11”）与来自发光二极管（LED）的不同比例的红色光通量（RPF）和蓝色光通量（BPF）的关系。处理设置为单色光，包括100 R：0 B %和0 R：100 B %，以及复合光（CL），包括75 R：25 B %、50 R：50 B %和25 R：75 B %。所有处理的总光通量设定为550 μmolm−2s−1。随着BPF的增加，油菜叶片由皱叶、边缘下卷向平叶、边缘略上卷转变，栅栏组织紧密度增加。100 R：0 B %处理的栅栏组织细胞仅为一层，其余处理的栅栏组织细胞均为两层。与100 R：0 B %相比，0 R：100 B %提高了叶片厚度、单位面积叶重（LMA）、气孔密度、单位重量叶绿素（Chl）含量和光合能力（Pmax）等指标，且高BPF比的CL提高了这些指标。而100 R：0 B %和高RPF比的CLs则提高了净光合速率（Pn）。CL处理下的叶片表现出较强的生长活力，而100 R：0 B %和0 R：100 B %处理下的叶片表现出较低的Fv/Fm（光合最大量子效率）和较高的H_2O_2含量。在100 R：0 B %或0 R：100 B %处理下，顶二叶表现出抗氧化酶活性高的抗逆反应，而顶三叶表现出不可逆的损伤和抗氧化酶活性的失活。结果表明，油菜叶片在0 R：100 B %或高BPF比的CL下表现出较高的光通量利用能力，而在100 R：0 B %或低BPF比的CL下表现出较高的光通量利用效率。在不同的R：B光通量比下，红光和蓝光在Pn中可能相互作用。当蓝光占优势时，Pn表现出B-偏好性。当红光占优势时，Pn表现出R-偏好性。CL对油菜幼苗的光合速率有较好的调节作用。
Rapeseed (Brassica napus L.) is sensitive to light quality. The factory production of rapeseed seedlings for vegetable use and for transplanting in the field requires an investigation of the responses of rapeseed to light quality. This study evaluated the responses of the leaf of rapeseed (cv. “Zhongshuang 11”) to different ratios of red-photonflux (RPF) and blue-photonflux (BPF) from light emitting diodes (LEDs). The treatments were set as monochromatic lights, including 100R:0B% and 0R:100B%, and compound lights (CLs), including 75R:25B%, 50R:50B%, and 25R:75B%. The total photonflux in all of the treatments was set as 550 μmolm−2s−1. With an increase of BPF, the rapeseed leaves changed from wrinkled blades and down-rolled margins to flat blades and slightly up-rolled margins, and the compact degree of palisade tissue increased. One layer of the cells of palisade tissue was present under 100R:0B%, whereas two layers were present under the other treatments. Compared to 100R:0B%, 0R:100B% enhanced the indexes of leaf thickness, leaf mass per area (LMA), stomatal density, chlorophyll (Chl) content per weight and photosynthetic capacity (Pmax), and the CLs with high BPF ratios enhanced these indexes. However, the 100R:0B% and CLs with high RPF ratios enhanced the net photosynthetic rate (Pn). The leaves under the CLs showed growth vigor, whereas the leaves under 100R:0B% or 0R:100B% were stressed with a low Fv/Fm (photosynthetic maximum quantum yield) and a high content of and H2O2. The top second leaves under 100R:0B% or 0R:100B% showed stress resistance responses with a high activity of antioxidase, but the top third leaves showed irreversible damage and inactivity of antioxidase. Our results showed that the rapeseed leaves grown under 0R:100B% or CLs with a high BPF ratio showed higher ability to utilize high photonflux, while the leaves grown under 100R:0B% or CLs with a low BPF ratio showed higher efficiency in utilizing low photonflux. Under different R:B photonflux ratios, red and blue lights may play mutual roles in Pn. When the blue light dominated, the Pn showed a B-preference. When the red light dominated, the Pn showed an R-preference. Furthermore, CLs were suitable for the Pn of rapeseed seedlings.