Biodegradable Nerve Guide with Glial Cell Line-Derived Neurotrophic Factor Improves Recovery After Facial Nerve Injury in Rats.
Biodegradable Nerve Guide with Glial Cell Line-Derived Neurotrophic Factor Improves Recovery After Facial Nerve Injury in Rats.
复制标题
含有胶质细胞系衍生神经营养因子的可生物降解神经导管可改善大鼠面神经损伤后的恢复。
DOI：
10.1089/fpsam.2022.0346
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发表时间：
2023
影响因子：
2
通讯作者：
Marra,KaceyG
中科院分区：
文献类型：
--
作者：
Bengur,FuatBaris;Komatsu,Chiaki;Fedor,CarolineNadia;Loder,Shawn;Baker,JocelynS;Totwani,Aanchal;Irgebay,Zhazira;Nerone,WVincent;Solari,MarioG;Marra,KaceyG
背景：含有胶质细胞源性神经营养因子（GDNF）的生物工程神经导管通过充当再生支架支持面神经损伤后的恢复。目的：比较对照、空神经导管和GDNF条件下神经导管修复大鼠面神经横断后的功能、电生理和组织学结果。方法：大鼠面神经颊分支横断和一期修复，并分为（1）单纯横断和修复组，（2）空导管增强横断和修复组，（3）GDNF引导增强横断和修复组。每周测量搅拌运动记录。结果：GDNF引导组大鼠的标准化拂动幅度峰值最早，而对照组大鼠的标准化拂动幅度峰值最早，且随时间延长而逐渐降低。CMAP在GDNF引导物放置后显著更高。平均纤维表面积的目标肌肉，轴突计数的损伤分支，和雪旺细胞的数量是最高的GDNF guides.Conclusion：可生物降解的神经指南含有双壁GDNF微球增强面神经横断和一期修复后的恢复。
Background:Bioengineered nerve guides with glial cell line-derived neurotrophic factor (GDNF) support recovery after facial nerve injury by acting as regenerative scaffolds.Objective:To compare functional, electrophysiological, and histological outcomes after repair of rat facial nerve transection in control, empty nerve guide, and nerve guide with GDNF conditions.Methods:Rats underwent transection and primary repair of the buccal branch of the facial nerve and were divided into (1) transection and repair only, (2) transection and repair augmented with empty guide, (3) transection and repair augmented with GDNF-guide groups. Weekly measurements of the whisking movements were recorded. At 12 weeks, compound muscle action potentials (CMAPs) at the whisker pad were assessed, and samples were collected for histomorphometric analysis.Results:Rats in GDNF-guide group displayed the earliest peak in normalized whisking amplitude. CMAPs were significantly higher after GDNF-guide placement. Mean fiber surface area of the target muscle, axonal count of the injured branch, and the number of Schwann cells were highest with GDNF guides.Conclusion:The biodegradable nerve guide containing double-walled GDNF microspheres enhanced recovery after facial nerve transection and primary repair.