Ia determinants on human T-cell subsets defined by monoclonal antibody. Activation stimuli required for expression
Ia determinants on human T-cell subsets defined by monoclonal antibody. Activation stimuli required for expression
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Ia 由单克隆抗体定义的人类 T 细胞亚群的决定因素。
DOI：
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发表时间：
1979
影响因子：
15.3
通讯作者：
S. Schlossman
中科院分区：
文献类型：
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作者：
E. Reinherz;P. Kung;J. Pesando;J. Ritz;G. Goldstein;S. Schlossman
使用与Ia抗原框架反应的单克隆抗体检查了活化后出现在人T细胞上的Ia抗原的性质及其表达所需的刺激。使用针对整个 T 细胞群 (OKT3) 或 T 细胞诱导子亚群 (OKT4) 的单克隆抗体纯化 T 细胞。通过间接免疫荧光，表明人类 T 细胞群在静息状态下不含可检测到的 Ia+ 细胞。相反，超过 60% 的 T 细胞群在混合淋巴细胞培养物中同种激活后表达 Ia 抗原。此外，这些 Ia 抗原在 OKT4+ 和 OKT4- 亚群中表达。类似地，植物血凝素和刀豆球蛋白 A 诱导大约 20% 的外周 T 细胞表达 Ia 抗原，并且这些抗原的表达不限于任一 OKT4 亚群。相反，只有最大程度增殖为可溶性抗原的诱导T细胞群在被破伤风类毒素激活后才表达Ia抗原。因此，人Ia抗原在独特的T细胞亚群上的表达取决于所利用的激活刺激以及个体亚群对给定刺激作出反应的能力。其他研究表明，Ia 抗原在激活后出现在先前的 Ia-T 细胞上，并不是由一小部分 Ia+ T 细胞的克隆扩增引起的。
The nature of Ia antigens which appear on human T cells after activation and the stimuli required for their expression was examined utilizing a monoclonal antibody reactive with the Ia antigen framework. T cells were purified using monoclonal antibodies directed either at the entire T-cell population (OKT3) or the T-cell inducer subset (OKT4). By indirect immunofluorescence, it was shown that the human T- cell population contains no detectable Ia+ cells in the resting state. In contrast, in excess of 60% of the T-cell population expresses Ia antigen after alloactivation in the mixed lymphocyte culture. Moreover, these Ia antigens are expressed within both the OKT4+ and OKT4- subsets. Similarly, phytohemagglutinin and concanavalin A induced approximately 20% of peripheral T cells to express Ia antigen and the expression of these antigens is not restricted to either OKT4 subset. In contrast, only the inducer T-cell population which proliferates maximally to soluble antigen expresses Ia antigens after activation by tetanus toxoid. Thus, the expression of human Ia antigens on unique T- cell subsets depends upon the activation stimuli utilized and ability of the individual subset to respond to a given stimulus. Additional studies indicated that Ia antigens appear on previously Ia- T cells after activation and do not result from clonal expansion of a small subset of Ia+ T cells.