Development and application of a simple colorimetric assay reveals widespread distribution of sodium channel mutations in Thai populations of Aedes aegypti
Development and application of a simple colorimetric assay reveals widespread distribution of sodium channel mutations in Thai populations of Aedes aegypti
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DOI：
10.1016/j.actatropica.2008.08.004
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发表时间：
2008-10-01
期刊：
影响因子：
2.7
通讯作者：
Ranson, Hilary
中科院分区：
文献类型：
--
作者：
Rajatileka, Shavanthi;Black, William C.;Ranson, Hilary
登革热及其更严重的并发症登革热出血热和登革热休克综合征是热带和亚热带国家日益严重的公共卫生问题。在缺乏疫苗的情况下，大多数登革热控制方案严重依赖使用杀虫剂来瞄准伊蚊媒介。由于常规用于控制的杀虫剂数量有限，监测抗药性的存在是登革热预防方案的一个重要组成部分。拟除虫菊酯杀虫剂的目标是昆虫神经元上的电压门控钠通道。在拉丁美洲和亚洲的埃及伊蚊种群中，该蛋白1016位的替换与拟除虫菊酯和滴滴涕耐药性有关。在这里，我们报告了一种简单的比色法的发展，以检测这些突变的个体蚊子。对180Ae诊断试验的评价。来自泰国的埃及人发现Val1016Gly突变在全国各地都存在高频率。该检测不需要专门的设备，并将使对杀虫剂抗药性相关等位基因的监测能够常规地纳入登革热监测行动。(C)2008爱思唯尔B.V.保留所有权利。
Dengue fever and its more serious complications dengue haemorrhagic fever and dengue shock syndrome are growing public health problems in tropical and subtropical countries. In the absence of a vaccine, most dengue control programmes rely heavily on the use of insecticides to target the Aedes mosquito vectors. As a limited number of insecticides are routinely used in control, monitoring for the presence of resistance is an essential component of dengue prevention programmes. The pyrethroid insecticides target the voltage-gated sodium channel on the insects' neurons. Substitutions at residue 1016 of this protein have been associated with pyrethroid and DDT resistance in Aedes aegypti populations from Latin America and Asia. Here we report on the development of a simple colorimetric assay to detect these mutations in individual mosquitoes. Evaluation of this diagnostic assay on 180 Ae. aegypti individuals from Thailand revealed the presence of high frequencies of the Val1016Gly mutation throughout the country. The assay requires no specialised equipment and will enable monitoring for insecticide resistance associated alleles to be routinely incorporated into dengue surveillance operations. (C) 2008 Elsevier B.V. All rights reserved.