On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development.
On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development.
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DOI：
10.1016/j.devcel.2015.10.010
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发表时间：
2015-11-09
影响因子：
11.8
通讯作者：
Kirschner MW
中科院分区：
文献类型：
--
作者：
Peshkin L;Wühr M;Pearl E;Haas W;Freeman RM Jr;Gerhart JC;Klein AM;Horb M;Gygi SP;Kirschner MW
从受精卵到成体发育的生化解释需要了解胚胎发生过程中随时间表达的蛋白质和 RNA。我们对非洲爪蟾早期发育过程中的蛋白质和 mRNA 动态进行了全面的表征。令人惊讶的是，我们发现大多数蛋白质水平变化很小，并且重复的基因表达相似。虽然蛋白质和 mRNA 水平之间的相关性很差，但使用蛋白质合成和降解率参数化的质量作用动力学模型将蛋白质动力学回归为 RNA 动力学，并校正了初始蛋白质浓度。这项研究通过便捷的门户网站提供了~10K 蛋白质和~28K 转录本绝对水平的详细数据，这为发育生物学家提供了丰富的资源。它强调了母亲嫁妆的持久影响，令人惊讶地发现，仅降解驱动蛋白质水平变化的情况很少，并强调了转录在塑造胚胎蛋白质组动态方面的重要性。
A biochemical explanation of development from the fertilized egg to the adult requires an understanding of the proteins and RNAs expressed over time during embryogenesis. We present a comprehensive characterization of protein and mRNA dynamics across early development in Xenopus. Surprisingly, we find that most protein levels change little and duplicated genes are expressed similarly. While the correlation between protein and mRNA levels is poor, a mass action kinetics model parameterized using protein synthesis and degradation rates regresses protein dynamics to RNA dynamics, corrected for initial protein concentration. This study provides detailed data for absolute levels of ∼10K proteins and ∼28K transcripts via a convenient web portal, a rich resource for developmental biologists. It underscores the lasting impact of maternal dowry, finds surprisingly few cases where degradation alone drives a change in protein level, and highlights the importance of transcription in shaping the dynamics of the embryonic proteome.