Synchronous activity of inhibitory networks in neocortex requires electrical synapses containing Connexin36
Synchronous activity of inhibitory networks in neocortex requires electrical synapses containing Connexin36
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DOI：
10.1016/s0896-6273(01)00373-7
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发表时间：
2001-08-16
期刊：
影响因子：
16.2
通讯作者：
Paul, DL
中科院分区：
文献类型：
--
作者：
Deans, MR;Gibson, JR;Paul, DL
抑制性中间神经元经常产生同步活动，作为其相互连接的一种新兴特性。为了确定电突触在这种活动中的作用，我们构建了表达组织化学报告基因代替差距连接蛋白Cx 36的小鼠。用生长抑素和小清蛋白定位报告基因表明，Cx36主要由中间神经元表达。电突触在对照组的皮质中间神经元中很常见，但在敲除组中几乎不存在。代谢型谷氨酸受体激动剂兴奋LTS中间神经元，在野生型和基因敲除动物的周围神经元中产生节律性抑制电位。然而，这些节奏的同步性较弱，在敲除中受到更多的空间限制。我们的结论是，电突触含有Cx36的广泛的，同步的抑制活性的产生是至关重要的。
Inhibitory interneurons often generate synchronous activity as an emergent property of their interconnections. To determine the role of electrical synapses in such activity, we constructed mice expressing histochemical reporters in place of the gap junction protein Cx36. Localization of the reporter with somatostatin and parvalbumin suggested that Cx36 was expressed largely by Interneurons. Electrical synapses were common among cortical interneurons in controls but were nearly absent in knockouts. A metabotropic glutamate receptor agonist excited LTS interneurons, generating rhythmic inhibitory potentials in surrounding neurons of both wild-type and knockout animals. However, the synchrony of these rhythms was weaker and more spatially restricted in the knockout. We conclude that electrical synapses containing Cx36 are critical for the generation of widespread, synchronous inhibitory activity.