BIOCHEMICAL AND HISTOCHEMICAL-LOCALIZATION OF MONOTERPENE BIOSYNTHESIS IN THE GLANDULAR TRICHOMES OF SPEARMINT (MENTHA-SPICATA)
BIOCHEMICAL AND HISTOCHEMICAL-LOCALIZATION OF MONOTERPENE BIOSYNTHESIS IN THE GLANDULAR TRICHOMES OF SPEARMINT (MENTHA-SPICATA)
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DOI：
10.1104/pp.89.4.1351
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发表时间：
1989-04-01
期刊：
影响因子：
7.4
通讯作者：
CROTEAU, R
中科院分区：
文献类型：
--
作者：
GERSHENZON, J;MAFFEI, M;CROTEAU, R
在薄荷（Mentha spicata）的腺毛中积累的初级单萜是酮(-)-香芹酮，它是由C10类异戊二烯中间体焦磷酸香叶基环化成烯烃(-)-柠檬烯，羟基化成(-)-反式香芹醇，然后脱氢形成的。对腺毛的选择性提取表明，基本上所有的环化酶和羟化酶活性都位于这些结构中，而卡维罗脱氢酶只有约30%位于这里，其余位于叶片的其余部分。组织化学方法证实了卡维罗脱氢酶活性的这种分布。从腺体和叶片的提取物中提取的部分纯化的卡维罗脱氢酶的电泳分析表明，在腺毛中存在一种独特的卡维罗脱氢酶，这表明在整个叶片中发现的其他脱氢酶可能只是将卡维罗作为一种不确定的底物。这些结果表明，香芹酮的生物合成只发生在这种天然产物积累的腺毛中。
The primary monoterpene accumulated in the glandular trichomes of spearmint (Mentha spicata) is the ketone (-)-carvone which is formed by cyclization of the C10 isoprenoid intermediate geranyl pyrophosphate to the olefin (-)-limonene, hydroxylation to (-)-trans-carveol and subsequent dehydrogenation. Selective extraction of the contents of the glandular trichomes indicated that essentially all of the cyclase and hydroxylase activities resided in these structures, whereas only about 30% of the carveol dehydrogenase was located here with the remainder located in the rest of the leaf. This distribution of carveol dehydrogenase activity was confirmed by histochemical methods. Electrophoretic analysis of the partially purified carveol dehydrogenase from extracts of both the glands and the leaves following gland removal indicated the presence of a unique carveol dehydrogenase species in the glandular trichomes, suggesting that the other dehydrogenase found throughout the leaf probably utilizes carveol only as an adventitious substrate. These results demonstrate that carvone biosynthesis takes place exclusively in the glandular trichomes in which this natural product accumulates.