MOLECULAR-ORGANIZATION OF JUNIN VIRUS-S RNA - COMPLETE NUCLEOTIDE-SEQUENCE, RELATIONSHIP WITH OTHER MEMBERS OF THE ARENAVIRIDAE AND UNUSUAL SECONDARY STRUCTURES
MOLECULAR-ORGANIZATION OF JUNIN VIRUS-S RNA - COMPLETE NUCLEOTIDE-SEQUENCE, RELATIONSHIP WITH OTHER MEMBERS OF THE ARENAVIRIDAE AND UNUSUAL SECONDARY STRUCTURES
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DOI：
10.1099/0022-1317-72-9-2129
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发表时间：
1991-09-01
影响因子：
3.8
通讯作者：
ROMANOWSKI, V
中科院分区：
文献类型：
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作者：
GHIRINGHELLI, PD;RIVERAPOMAR, RV;ROMANOWSKI, V
在这项研究中，产生了覆盖胡宁病毒整个 S RNA 分子的重叠 cDNA 克隆，胡宁病毒是一种引起阿根廷出血热的沙粒病毒。 使用双脱氧核苷酸链终止法测定了该 3400 个核苷酸 RNA 的完整序列。 核衣壳蛋白（N）和糖蛋白前体（GPC）基因被鉴定为两个极性相反的非重叠开放阅读框，分别编码564和481个氨基酸的初级翻译产物。 后者的细胞内加工产生病毒包膜中的糖蛋白。 胡宁病毒N蛋白与其他沙粒病毒同源蛋白的比较表明，氨基酸序列比较保守，同一性在46%~76%之间。 GPC 的 N 端一半表现出更高程度的保守性（54% 至 82%），而 C 端一半则保守程度较低（21% 至 50%）。 在所有比较中，当胡宁病毒和塔卡里布病毒序列比对时，看到了最高水平的氨基酸序列同一性。 胡宁病毒 S RNA 5' 端的核苷酸序列与其他已测序沙粒病毒的核苷酸序列不同。 然而，它与 3' 端序列互补，并可能形成非常稳定的狭长结构 (DELTA-G -242.7 kJ/mol)，涉及 N 和 GPC 基因上游的完整非编码区。 此外，在编码序列下游的基因间区域中发现了独特的二级结构；胡宁病毒 S RNA 显示出由两个发夹环（DELTA-G - 163.2 和 -239.3 kJ/mol）组成的潜在二级结构，而不是其他沙粒病毒中常见的单个发夹环。 讨论了沙粒病毒 S RNA 核苷酸序列及其编码产物的结构和功能分析。
In this study, overlapping cDNA clones covering the entire S RNA molecule of Junin virus, an arenavirus that causes Argentine haemorrhagic fever, were generated. The complete sequence of this 3400 nucleotide RNA was determined using the dideoxynucleotide chain termination method. The nucleocapsid protein (N) and the glycoprotein precursor (GPC) genes were identified as two non-overlapping open reading frames of opposite polarity, encoding primary translation products of 564 and 481 amino acids, respectively. Intracellular processing of the latter yields the glycoproteins found in the viral envelope. Comparison of the Junin virus N protein with the homologous proteins of other arenaviruses indicated that amino acid sequences are conserved, the identity ranging from 46 to 76%. The N-terminal half of GPC exhibits an even higher degree of conservation (54 to 82%), whereas the C-terminal half is less conserved (21 to 50%). In all comparisons the highest level of amino acid sequence identity was seen when Junin virus and Tacaribe virus sequences were aligned. The nucleotide sequence at the 5' end of Junin virus S RNA is not identical to that determined of the other sequenced arenaviruses. However, it is complementary to the 3'-terminal sequences and may form a very stable panhandle structure (DELTA-G -242.7 kJ/mol) involving the complete non-coding regions upstream from both the N and GPC genes. In addition, a distinct secondary structure was identified in the intergenic region, downstream from the coding sequences; Junin virus S RNA shows a potential secondary structure consisting of two hairpin loops (DELTA-G - 163.2 and -239.3 kJ/mol) instead of the single hairpin loop that is usually found in other arenaviruses. The analysis of the arenavirus S RNA nucleotide sequences and their encoded products is discussed in relation to structure and function.