Nuclear and mitochondrial DNA analyses reveal four genetically separated breeding units of the swordfish
Nuclear and mitochondrial DNA analyses reveal four genetically separated breeding units of the swordfish
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DOI：
10.1111/j.1095-8649.2000.tb02125.x
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发表时间：
2000-05-01
影响因子：
2
通讯作者：
Takeyama, H
中科院分区：
文献类型：
--
作者：
Chow, S;Takeyama, H
利用限制性片段长度多态性（RFLP）和核苷酸序列分析方法，对剑吻剑鱼（Xiphias gladius）钙调素基因内含子4（CaM）和线粒体DNA D环区（D-loop）的变异进行了研究。在8个样本（地中海、塔里法、4个大西洋地区、印度洋和太平洋）共567个个体中，在CaM基因座上观察到两个等位基因（A和B）。该位点的基因型分布符合Hardy-Weinberg平衡。地中海-西北大西洋样品（A=0.347-0.493）与热带大西洋和印度洋-太平洋样品（A=0.81-1）之间的等位基因频率存在显著差异。在D-loop区，Alu Ⅰ和Rsa Ⅰ双酶切分别检测到4种（A-D）和8种（A-H）基因型。印度-太平洋样本中Alu I的C型频率（0.2）低于大西洋和地中海样本（0.365-0.643）。Rsa I的C型频率从太平洋（0.398）到西北大西洋（0.698）和塔里法（0.949）样本增加，地中海样本固定为C型。这些样本被分为三个组（地中海-塔里法，大西洋和印度洋-太平洋）使用的mtDNA基因型分布的异质性检验。CaM和D-loop位点的联合分析表明，至少有四个繁殖单位：地中海，西北大西洋，热带南大西洋，印度洋-太平洋。(C)2000年，不列颠群岛渔业协会。
Variants in the calmodulin gene intron 4 (CaM) and the mtDNA D-loop region (D-loop) detected by RFLP and nucleotide sequence analyses were used to investigate the global stock structure of the swordfish Xiphias gladius. Two alleles (A and B) were observed at the CaM locus among 8 samples (Mediterranean, Tarifa, four Atlantic areas, Indian and Pacific oceans) comprising 567 individuals. Genotype distributions at this locus within samples were in accordance with Hardy-Weinberg equilibrium. Significant differences in allele frequencies were observed between Mediterranean-Northwest Atlantic samples (A=0.347-0.493) and those from tropical Atlantic and Indo-Pacific (A=0.81-1). In the D-loop region, Alu I and Rsa I digestions detected 4 (A-D) and 8 (A-H) genotypes, respectively. The frequency of type C for Alu I was lower in Indo-Pacific samples (0.2) than Atlantic and Mediterranean samples (0.365-0.643). Frequency of type C for Rsa I increased from the Pacific (0.398) to the Northwest Atlantic (0.698) and Tarifa (0.949) samples, and the Mediterranean sample was fixed for type C. These samples were classified into three groups (Mediterranean-Tarifa, Atlantic and Indo-Pacific) using a heterogeneity test of mtDNA genotype distributions. Combined analysis of the CaM and D-loop loci indicated that there are at least four breeding units: Mediterranean, northwestern Atlantic, tropical to South Atlantic, and Indo-Pacific. (C) 2000 The Fisheries Society of the British Isles.