Structural transitions in polyribocytidylic acid induced by changes in pH and temperature: vibrational circular dichroism study in solution and film states.
Structural transitions in polyribocytidylic acid induced by changes in pH and temperature: vibrational circular dichroism study in solution and film states.
复制标题
DOI：
10.1021/jp063221l
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Ana G. Petrovic;P. Polavarapu
中科院分区：
文献类型：
--
作者：
Ana G. Petrovic;P. Polavarapu
本文报道了聚核糖酸（polyC）在D（2）O缓冲溶液中的振动吸收光谱和振动圆二色性（VCD）光谱随pH和温度的变化。对这些光谱数据的分析使我们确定了在大约1693 cm（-1）处的吸收带和相关的负VCD偶联作为polyC双螺旋形式的诊断标志物。另一种解释表明四链体形成聚C也进行了讨论。除了溶液状态的光谱数据，pH值依赖的吸收和VCD光谱的polyC薄膜来自稀H（2）O解决方案也被提出。聚C膜的吸收光谱和VCD光谱的pH值依赖性的变化被发现是类似的聚C溶液中观察到的。
Changes in vibrational absorption and vibrational circular dichroism (VCD) spectra of polyribocytidylic acid (polyC) in buffered D(2)O solution as a function of pH and temperature are reported. Analysis of these spectral data led us to establish the absorption band at approximately 1693 cm(-1) and associated negative VCD couplet as diagnostic markers of the double-helical form of polyC. An alternate interpretation suggesting quadruplex formation for polyC is also discussed. In addition to the solution state spectral data, pH-dependent absorption and VCD spectra for polyC films derived from dilute H(2)O solutions are also presented. The pH-dependent changes in the absorption and VCD spectra of polyC films are found to be similar to those observed for polyC in solution.