EXPRESSION OF THE NEU PROTOONCOGENE BY SCHWANN-CELLS DURING PERIPHERAL-NERVE DEVELOPMENT AND WALLERIAN DEGENERATION
EXPRESSION OF THE NEU PROTOONCOGENE BY SCHWANN-CELLS DURING PERIPHERAL-NERVE DEVELOPMENT AND WALLERIAN DEGENERATION
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DOI：
10.1002/jnr.490310406
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发表时间：
1992-04-01
影响因子：
4.2
通讯作者：
SCHERER, SS
中科院分区：
文献类型：
--
作者：
COHEN, JA;YACHNIS, AT;SCHERER, SS
neu基因编码一种假定的酪氨酸激酶生长因子受体（称为p185 neu），最初被确定为通过用乙基亚硝基脲经胎盘处理大鼠胚胎诱导的神经胶质瘤和神经鞘瘤中的显性转化基因。本研究旨在确定neu基因在周围神经中的表达模式。从大鼠坐骨神经中分离的总RNA的北方印迹分析表明，在出生后第1天和第7天显著的neu mRNA表达，直到成年表达显著降低。免疫组织化学研究证实了p185 neu的表达的雪旺细胞（SC）在发展中的坐骨神经和最低限度的p185 neu免疫反应在成人神经。然而，neu mRNA和p185 neu蛋白进行性增加坐骨神经切断后，在成年动物。此外，neu mRNA和p185 neu在新生大鼠坐骨神经SC和几个SC衍生的细胞系中被发现。在静息SC中，neu mRNA以低水平表达，但通过用毛喉素和胶质生长因子处理而大大增加。这些研究表明neu基因及其蛋白产物p185neu在体内和体外均由SC表达，并表明p185neu在SC增殖或分化的调节中起作用。
The neu gene, which encodes a putative tyrosine kinase growth factor receptor termed p185neu, was originally identified as a dominant transforming gene in neurogliomas and schwannomas induced by transplacental treatment of rat embryos with ethylnitrosourea. The present studies were undertaken to determine the expression pattern of the neu gene in peripheral nerve. Northern blot analysis of total RNA isolated from rat sciatic nerves demonstrated prominent neu mRNA expression on postnatal days 1 and 7, with substantially lower expression up to adulthood. Immunohistochemical studies confirmed expression of p185neu by Schwann cells (SC) in developing sciatic nerve and minimal p185neu immunoreactivity in adult nerves. However, neu mRNA and p185neu protein progressively increased following sciatic nerve transection in adult animals. In addition, neu mRNA and p185neu were found in neonatal rat sciatic nerve SC and several SC-derived cell lines. In resting SC, neu mRNA was expressed at a low level, but was greatly increased by treatment with forskolin and glial growth factor. These studies demonstrate that the neu gene and its protein product, p185neu, are expressed by SC both in vivo and in vitro and suggest that p185neu plays a role in the regulation of SC proliferation or differentiation.