Fractionation of normal and leukemic T-cells by lectin-affinity column chromatography.
Fractionation of normal and leukemic T-cells by lectin-affinity column chromatography.
复制标题
通过凝集素亲和柱层析分离正常和白血病 T 细胞。
DOI：
10.1016/s0304-3835(02)00207-0
复制
发表时间：
2002
期刊：
影响因子：
9.7
通讯作者：
O. Nakamura
中科院分区：
文献类型：
--
作者：
H. Ohba;R. Bakalova;S. Moriwaki;O. Nakamura
A method for rapid fractionation of normal and leukemic T-cells (Jurkat, RPMI-8402, MOLT-4), using lectin-affinity column chromatography, is described. CNBr-activated Sepharose 6MB was used as a non-mobile phase. The gel was covalently conjugated with Dolichos biflorus agglutinin (DBA) over 24 h. The normal cells were eluted by phosphate buffered saline (Ca2+and Mg2+free), while the leukemic T-cells, interacting with DBA, were removed by N-acetyl-d-galactosamine or by low-concentrated acetic acid as a mobile phase. The cell fractions were detected spectrophotometrically at 600 nm. The rate of cell elution decreased in the order: normal>leukemic T-cells. The viability and the type of separated T-cell fractions were characterized by flow cytometry, using adequate fluorescent antibodies. The interactions between leukemic T-cells and DBA-saturated Sepharose beads were examined by fluorescent microscopy, using fluorescent isothiocyanate-DBA as a fluorescent marker.
来自正常人和慢性白血病白细胞的天冬酰胺连接寡糖的凝集素亲和力分级。
DOI：
--
发表时间：
1987
期刊：
Cancer biochemistry biophysics
影响因子：
--
作者：
Forsberg,LS;Macher,BA
通讯作者：
Macher,BA