High detection frequency and viral loads of human rhinovirus species A to C in fecal samples; diagnostic and clinical implications
High detection frequency and viral loads of human rhinovirus species A to C in fecal samples; diagnostic and clinical implications
复制标题
DOI：
10.1002/jmv.23203
复制
发表时间：
2012-03-01
影响因子：
12.7
通讯作者：
Simmonds, P.
中科院分区：
文献类型：
--
作者：
Harvala, H.;McIntyre, C. L.;Simmonds, P.
人类鼻病毒（HRV）可分为三个种类： HRV-A 至 HRV-C。迄今为止，已鉴定出多达 148 种不同的 HRV（血清）类型。由于 HRV 和肠道病毒 (EV) 5'-NCR 之间的序列相似性，设计 EV 特异性 RT-PCR 检测是有问题的。本研究的目的是评估 EV RT-PCR 对不同鼻病毒的误检率，并评估这种交叉反应的诊断和临床意义。通过分光光度法对从 cDNA 模板创建的 HRV A-C 的体外 RNA 转录本进行定量。该研究纳入了作为 EV 常规诊断一部分的 621 份粪便样本、17 份转介进行分型的 EV 阳性粪便样本、288 份提交用于胃肠炎调查的粪便样本以及 1,500 份脑脊液样本。 EV 特异性 RT-PCR 检测到 HRV-A1b、HRV-B14 和 HRV-Crpat18 的 RNA 转录本，但与 EV 转录本相比，灵敏度降低了 101,000。通过 EV RT-PCR 筛查粪便样本，发现 13 个阳性样本，随后被鉴定为鼻病毒；通过巢式 HRV RT-PCR 鉴定了另外 26 个 HRV 阳性样本。所有患者均住院治疗，且大多出现腹泻症状。总共确定了 26 种 HRV 类型（HRV-A：46%；HRV-B：13%；HRV-C：41%）。结果证实，EV 特异性 RT-PCR 可以检测 HRV，并且在实际水平上，如果粪便样本用于疑似病毒性脑膜炎病例的替代筛查，则可以识别潜在的解释问题。粪便样本中的高检测频率（10%）和病毒载量为 HRV 肠道复制提供了证据，其与肠道疾病的关联需要进一步的病因学研究。 J. Med。病毒。 84:536542, 2012。(C) 2011 Wiley 期刊公司。
Human rhinoviruses (HRVs) can be divided into three species; HRV-A to HRV-C. Up to 148 different HRV (sero)types have been identified to date. Because of sequence similarity between 5'-NCR of HRVs and enteroviruses (EVs), it is problematic to design EV-specific RT-PCR assays. The aims of this study were to assess the rate of false-detection of different rhinoviruses by EV RT-PCR, and to evaluate the diagnostic and clinical significance of such cross-reactivity. In vitro RNA transcripts of HRV A-C created from cDNA templates were quantified spectrophotometrically. Six hundred twenty-one stool samples screened as part of routine diagnostic for EV, 17 EV-positive stool samples referred for typing, 288 stool samples submitted for gastroenteritis investigations, and 1,500 CSF samples were included in the study. EV-specific RT-PCR detected RNA transcripts of HRV-A1b, HRV-B14, and HRV-Crpat18 but with 101,000 reduced sensitivity compared to EV transcripts. Screening fecal samples by EV RT-PCR identified 13 positive samples identified subsequently as rhinoviruses; a further 26 HRV-positive samples were identified by nested HRV RT-PCR. All individuals were hospitalized and presented mostly with diarrhea. A total of 26 HRV types were identified (HRV-A: 46%; HRV-B: 13%; HRV-C: 41%). Results confirm that EV-specific RT-PCR can detect HRVs, and at a practical level, identify potential problems of interpretation if fecal samples are used for surrogate screening in cases of suspected viral meningitis. High detection frequencies (10%) and viral loads in stool samples provide evidence for enteric replication of HRV, and its association with enteric disease requires further etiological studies. J. Med. Virol. 84:536542, 2012. (C) 2011 Wiley Periodicals, Inc.