Genomic instability and increased expression of BUB1B and MAD2L1 genes in ductal breast carcinoma
Genomic instability and increased expression of BUB1B and MAD2L1 genes in ductal breast carcinoma
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DOI：
10.1016/j.canlet.2007.03.021
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发表时间：
2007-09-08
期刊：
影响因子：
9.7
通讯作者：
Parrella, Paola
中科院分区：
文献类型：
--
作者：
Scintu, Marina;Vitale, Rita;Parrella, Paola
在一系列浸润性导管乳腺癌中，我们通过荧光原位杂交研究了染色体和染色体内不稳定性的状态，并确定了参与有丝分裂纺锤体检查点途径的两个基因BUBIB和MAD 2L 1的mRNA表达水平。所有乳腺癌标本的染色体不稳定率（平均56.86%，范围36.24-76.78%）均高于对照组（平均11.54%，范围9.91-14.84%）（P < 0.0001）。染色体内不稳定性在肿瘤组（平均18.45%，范围8.34-35.8%）明显高于对照组（平均4.18%，范围3.47-4.81%）（P < 0.0001）。在大多数测试的肿瘤中证实BUBIB和MAD 2L 1转录物增加。BUBIB mRNA水平而非MAD 2L 1水平与染色体内不稳定性相关（r = 0.722，P = 0.018）。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
In a series of invasive ductal breast carcinoma, we investigated the status of chromosomal and intrachromosomal instability by fluorescence in situ hybridisation and determined the level of mRNA expression for two genes involved in the mitotic spindle checkpoint pathway, BUBIB and MAD2L1. All breast cancers demonstrated higher chromosomal instability rates in tumor samples (average: 56.86%, range: 36.24-76.78%) than in controls (average: 11.54%, range: 9.91-14.84%) (P < 0.0001). As well as intrachromosomal instability rates were elevated in tumor (average: 18.45% range: 8.34-35.8%) as compared with controls (average: 4.18% range: 3.47-4.81%) (P < 0.0001). An increase in BUBIB and MAD2L1 transcripts was demonstrated in the majority of the tumor tested. BUBIB mRNA levels but not MAD2L1 levels correlated with intrachromosomal instability (r = 0.722, P = 0.018). (c) 2007 Elsevier Ireland Ltd. All rights reserved.