Effect of interferon and double-stranded RNA on B-cell function in mouse islets of Langerhans.
Effect of interferon and double-stranded RNA on B-cell function in mouse islets of Langerhans.
复制标题
干扰素和双链 RNA 对小鼠胰岛 B 细胞功能的影响。
DOI：
10.1042/bj2280087
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
K. Taylor
中科院分区：
文献类型：
--
作者：
C. Rhodes;K. Taylor;K. Taylor
α干扰素和β干扰素对离体小鼠胰岛的直接作用在体外被研究，发现是相似的。干扰素浓度高于350单位/ml孵育7小时后，葡萄糖刺激（pro）胰岛素的生物合成被显著抑制，仅对总蛋白的生物合成有轻微抑制。在抗干扰素抗体的额外存在下，抑制可以被消除。干扰素不影响胰岛素释放、总胰岛素含量或胰岛的葡萄糖氧化。腺苷、d -甘油醛、甘露糖、n -乙酰氨基葡萄糖和亮氨酸对（原）胰岛素生物合成的刺激也被干扰素抑制，对胰岛素释放无影响。在诱导干扰素的dsRNA（双链RNA）浓度（1-100微克/毫升）下，葡萄糖刺激（pro）胰岛素的生物合成被抑制，但不会显著影响胰岛素的释放。dsRNA本身可能抑制受刺激的胰岛素生物合成，也可能通过诱导干扰素间接起作用。
The direct effects of alpha- and beta-interferons on isolated mouse pancreatic islets were investigated in vitro and found to be similar. After 7 h incubation with interferon concentrations above 350 units/ml, glucose-stimulated (pro)insulin biosynthesis was significantly inhibited, with only a slight inhibition of total protein biosynthesis. Inhibition could be abolished in the additional presence of an anti-interferon antibody. Interferon did not affect insulin release, total insulin content, or glucose oxidation of the islets. The stimulation of (pro)insulin biosynthesis by adenosine, D-glyceraldehyde, mannose, N-acetylglucosamine and leucine was also inhibited by interferon, with no effect on insulin release. At concentrations of dsRNA (double-stranded RNA) said to induce interferon (1-100 micrograms/ml), glucose-stimulated (pro)insulin biosynthesis was inhibited without significantly affecting insulin release. The dsRNA may itself inhibit stimulated (pro)insulin biosynthesis or may function indirectly by the induction of interferon.