New technologies for the detection of circulating tumour cells
New technologies for the detection of circulating tumour cells
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DOI：
10.1093/bmb/ldq011
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发表时间：
2010-06-01
影响因子：
6.7
通讯作者：
Jabado, Nada
中科院分区：
文献类型：
--
作者：
Gerges, Noha;Rak, Janusz;Jabado, Nada
绝大多数与癌症相关的死亡是由于原发肿瘤的转移性扩散。循环肿瘤细胞（CTC）对于建立转移是必不可少的，并且它们的检测长期以来被认为是评估给定肿瘤的侵袭性及其随后在远处器官生长的潜力的可能工具。传统的标记物在检测隐匿性转移方面并不可靠，例如，无法识别需要更积极或更好调整治疗的癌症患者的40%。最近在转移性乳腺癌中的研究表明，CTC检测可用作总生存期和评估治疗反应的标志物。CTC检测在早期乳腺癌和其他实体瘤中的益处需要进一步验证。此外，最佳的CTC检测技术是争议的主题，因为一些缺乏再现性、灵敏度和/或特异性。最近的技术进步允许在血液或骨髓中的单细胞水平上检测和表征CTC。它们的可重复性推动了CTC在几项大型临床试验中用于癌症分期和系统性抗癌治疗的实时监测。CTC检测正在被整合到大型临床试验中，以确定其在癌症患者管理中的潜力，并提高我们对转移生物学的理解。该审查将侧重于目前使用的技术，所取得的技术进步，四氯化碳检测的局限性和在这一领域的未来前景。
The vast majority of cancer-related death is due to the metastatic spread of the primary tumour. Circulating tumour cells (CTC) are essential for establishing metastasis and their detection has long been considered as a possible tool to assess the aggressiveness of a given tumour and its potential of subsequent growth at distant organs. Conventional markers are not reliable in detecting occult metastasis and, for example, fail to identify similar to 40% of cancer patients in need of more aggressive or better adjusted therapies. Recent studies in metastatic breast cancer have shown that CTC detection can be used as a marker for overall survival and assessment of the therapeutic response. The benefits of CTC detection in early breast cancer and other solid tumours need further validation. Moreover, optimal CTC detection techniques are the subject of controversy as several lack reproducibility, sensitivity and/or specificity. Recent technical advances allow CTC detection and characterization at the single-cell level in the blood or in the bone marrow. Their reproducibility propels the use of CTC in cancer staging and real-time monitoring of systemic anticancer therapies in several large clinical trials. CTC assays are being integrated in large clinical trials to establish their potential in the management of cancer patients and improve our understanding of metastasis biology. This review will focus on the techniques currently used, the technical advancements made, the limitations of CTC detection and future perspectives in this field.