DEC2 expression antagonizes cisplatin‑induced apoptosis in human esophageal squamous cell carcinoma.
DEC2 expression antagonizes cisplatin‑induced apoptosis in human esophageal squamous cell carcinoma.
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DOI：
10.3892/mmr.2017.6571
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发表时间：
2017-07
影响因子：
3.4
通讯作者：
Kijima H
中科院分区：
文献类型：
--
作者：
Sato H;Wu Y;Kato Y;Liu Q;Hirai H;Yoshizawa T;Morohashi S;Watanabe J;Kijima H
分化胚胎软骨细胞表达基因1（DEC 1）和分化胚胎软骨细胞表达基因2（DEC 2）属于碱性螺旋-环-螺旋因子的Hairy/Enhancer of Split亚家族。以往的研究表明，DEC蛋白参与昼夜节律的调节，对缺氧的反应和肿瘤的发生。然而，DEC 1和DEC 2在食管癌细胞凋亡中的作用尚不清楚。在本研究中，凋亡相关标志物在人食管鳞癌TE-11细胞与顺铂处理的表达的变化进行了检查，而整体细胞活力和凋亡进行了分析MTS测定和苏木精和伊红染色，分别。顺铂治疗后，DEC 2的表达下调，而DEC 1的表达上调。在顺铂治疗期间DEC 2过表达显著抑制促凋亡因子Bim的表达，并轻微增加抗凋亡因子Bcl-xL。然而，在顺铂治疗期间DEC 1的过表达未能影响这些标志物的表达。此外，DEC 2的过表达提高了细胞活力，减少了顺铂诱导的细胞凋亡。这些结果表明DEC 2在TE-11食管鳞状细胞癌细胞中具有抗凋亡作用。因此，抑制DEC 2与顺铂联合治疗食管癌可能具有治疗潜力。
Differentiated embryonic chondrocyte expressed gene 1 (DEC1) and differentiated embryonic chondrocyte expressed gene 2 (DEC2) belong to the Hairy/Enhancer of Split subfamily of basic helix-loop-helix factors. Previous studies have demonstrated that DEC proteins are involved in the regulation of circadian rhythms, response to hypoxia, and tumorigenesis. However, the roles of DEC1 and DEC2 in apoptosis of esophageal carcinoma remain unclear. In the present study, alterations in expression of apoptosis-related markers in human esophageal squamous cell carcinoma TE-11 cells treated with cisplatin were examined by western blot, while overall cell viability and apoptosis were analyzed by MTS assay and hematoxylin and eosin staining, respectively. Following cisplatin treatment, expression of DEC2 was downregulated, whereas expression of DEC1 was upregulated. DEC2 overexpression during cisplatin treatment markedly inhibited expression of the pro-apoptotic factor Bim and slightly increased the anti-apoptotic factor Bcl-xL. However, overexpression of DEC1 during cisplatin treatment failed to affect expression of these markers. Additionally, overexpression of DEC2 improved cell viability and decreased cell apoptosis induced by cisplatin. These results suggested that DEC2 exhibits anti-apoptotic effects in TE-11 esophageal squamous cell carcinoma cells. Inhibiting DEC2 may therefore have therapeutic potential for the treatment of esophageal cancer, in combination with cisplatin.