Addition of metal chlorides to a HOCl conditioner can enhance bond strength to smear layer deproteinized dentin
Addition of metal chlorides to a HOCl conditioner can enhance bond strength to smear layer deproteinized dentin
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在 HOCl 调理剂中添加金属氯化物可以增强涂片层脱蛋白牙本质的粘合强度
DOI：
10.1016/j.dental.2022.06.006
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发表时间：
2022
期刊：
影响因子：
5
通讯作者：
Nakajima Masatoshi
中科院分区：
文献类型：
--
作者：
Sanon Kittisak;Tichy Antonin;Hatayama Takashi;Thanatvarakorn Ornnicha;Prasansuttiporn Taweesak;Wada Takahiro;Shimada Yasushi;Hosaka Keiichi;Nakajima Masatoshi
方法用105ppm的次氯酸溶液(含/不含氯化锶0.05M、0.1M、0.2M、0.4M)或氯化锌(0.05M、0.1M、0.2M、0.2M)对有标准涂膜层的人牙本质表面进行脱蛋白处理，分别处理S、S、S 5、15、30岁。用水洗去蛋白溶液5、15、30、S、S、S。用一步自酸蚀粘结剂Bond Force II或通用粘合剂Clearfi Universal Bond Quick粘接处理后的表面，24小时后测量µTBS。此外，通过衰减全反射傅里叶变换红外光谱测量酰胺：磷酸盐比例的变化，比较有/无金属氯化物的HOCl溶液的脱蛋白效果。统计分析采用多因素方差分析、Tukey‘s后置检验和t检验(p<0.05)。结果用纯高氯预处理15℃的S和30℃的S显著降低了酰胺：磷的比例(p<0.05)，表明有效的脱蛋白，但只有当高氯清洗30℃时，两种粘结剂的uTBS才显著增加(p<0.05)。增加金属氯化物的浓度可以将洗出时间缩短到S的5%，同时保持改进的µTBS(p<0.05)。添加金属氯化物对高氯酸盐的脱蛋白效果没有显著影响(P&>0.05)。显著的是，添加金属氯化物可以提高高氯酸盐用于涂膜层脱蛋白的效果，因为它们的浓度增加，可以将洗出时间从S的30%缩短到S的5%。
ObjectivesTo evaluate the effect of smear layer deproteinization using hypochlorous acid (HOCl) with/without metal chlorides (SrCl2and ZnCl2) on the microtensile bond strength (µTBS) of two simplified adhesives to dentin.MethodsHuman dentin surfaces with a standardized smear layer were pretreated using a 105 ppm HOCl solution with/without SrCl2(0.05 M, 0.1 M, 0.2 M, 0.4 M) or ZnCl2(0.05 M, 0.1 M, 0.2 M) for 5 s, 15 s, or 30 s. After the deproteinizing solution was washed out with water for 5 s, 15 s, or 30 s, pretreated surfaces were bonded with one-step self-etch adhesive Bond Force II or universal adhesive Clearfil Universal Bond Quick, and µTBS was measured after 24 h. Additionally, the deproteinizing effects of HOCl solutions with/without the metal chlorides were compared by measuring changes in the amide:phosphate ratio using attenuated total reflection Fourier transform infrared spectroscopy. Statistical analysis was performed using multifactor ANOVA, Tukey’s post hoc tests and t-tests (p < 0.05).ResultsPretreatment with pure HOCl for 15 s and 30 s significantly decreased the amide:phosphate ratio (p < 0.05), indicating effective deproteinization, but the µTBS of both adhesives increased significantly only if HOCl was washed out for 30 s (p < 0.05). Increasing the concentrations of metal chlorides enabled shortening of the wash-out time down to 5 s while maintaining the improved µTBS (p < 0.05). The deproteinizing effect of HOCl was not significantly altered by the addition of metal chlorides (p > 0.05).SignificanceThe effectiveness of smear layer deproteinization using HOCl can be improved by the addition of metal chlorides, as their increasing concentration allowed to shorten the wash-out time from 30 s down to 5 s