siRNA targeted against matrix metalloproteinase 11 inhibits the metastatic capability of murine hepatocarcinoma cell Hca-F to lymph nodes (Retracted Article)
siRNA targeted against matrix metalloproteinase 11 inhibits the metastatic capability of murine hepatocarcinoma cell Hca-F to lymph nodes (Retracted Article)
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DOI：
10.1016/j.biocel.2007.05.023
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发表时间：
2007-01-01
影响因子：
4
通讯作者：
Zhang, Jianing
中科院分区：
文献类型：
--
作者：
Jia, Li;Wang, Shujing;Zhang, Jianing
基质金属蛋白酶-11（MMP-11）属于MMP家族的特殊成员，是一组参与肿瘤进展、侵袭和转移的锌依赖性内肽酶。 MMP-11 在肿瘤细胞和位于肿瘤附近的基质成纤维细胞中强烈表达。本研究通过 RNA 干扰 (RNAi) 方法研究了 MMP-11 表达在具有高度淋巴转移潜力的小鼠肝癌细胞系 Hca-F 中的可能作用。结果表明，针对MMP-11的小干扰RNA（siRNA）显着阻碍Hca-F细胞在软琼脂中的增殖和集落形成，并导致Hca-F细胞凋亡。 MMP-11 表达的减少还导致 Hca-F 细胞在体外和体内的迁移和粘附显着减少。此外，体内转移测定表明，Hca-F细胞中MMP-11表达的下调减弱了Hca-F细胞向外周淋巴结的转移潜力。这些数据共同为 MMP-11 的功能提供了令人信服的证据，并表明 MMP-11 作为肿瘤淋巴转移相关基因，并可能代表基因治疗的新潜在靶标。 (C) 2007 Elsevier Ltd. 保留所有权利。
Matrix metalloproteinase-11 (MMP-11) belongs to the particular member of MMP family, a group of zinc-dependent endopeptidases involved in tumor progression, invasion and metastasis. MMP-11 is strongly expressed in tumor cells and stromal fibroblasts located in the immediate vicinity of tumor. This study investigated the possible role of MMP-11 expression in mouse hepatocarcinoma cell line Hca-F with highly lymphatic metastasis potential by RNA interference (RNAi) approach. The results showed that a small interfering RNA (siRNA) targeted against MMP-11 significantly impeded Hca-F cells proliferation and colony formation in soft agar, as well as resulted in Hca-F cell apoptosis. This reduction of MMP-11 expression also led to the decreased migration and adhesion of Hca-F cells dramatically both in vitro and in vivo. Furthermore, in vivo metastasis assay indicated that down-regulation of MMP-11 expression in Hca-F cells attenuated the metastatic potential of Hca-F cells to peripheral lymph nodes. These data together provide compelling evidence into the function of MMP-11 and suggest that MMP-11 act as a tumor lymphatic metastasis-associated gene, and could represent a new potential target for gene therapy. (C) 2007 Elsevier Ltd. All rights reserved.