Expression of peptidylarginine deiminase-2 and -4, citrullinated proteins and anti-citrullinated protein antibodies in human gingiva
Expression of peptidylarginine deiminase-2 and -4, citrullinated proteins and anti-citrullinated protein antibodies in human gingiva
复制标题
DOI：
10.1111/jre.12002
复制
发表时间：
2013-04-01
影响因子：
3.5
通讯作者：
Bartold, P. M.
中科院分区：
文献类型：
--
作者：
Harvey, G. P.;Fitzsimmons, T. R.;Bartold, P. M.
背景与目的研究瓜氨酸化蛋白、肽基精氨酸脱亚胺酶2型（PAD-2）和肽基精氨酸脱亚胺酶4型（PAD-4）在牙周组织中的表达，确定抗环瓜氨酸化蛋白抗体（anti-CCP）在龈沟液（GCF）中的存在，并比较这些蛋白在炎症和非炎症部位的表达。材料和方法使用抗瓜氨酸化蛋白PAD-2和PAD-4的抗体对组织切片进行染色。采用RT-PCR方法检测炎症和非炎症牙龈组织中PAD-2和PAD-4 mRNA的表达。ELISA法检测龈沟液中抗CCP抗体。结果牙龈组织中检测到瓜氨酸化蛋白、PAD-2和PAD-4。炎症与这些蛋白的表达之间存在相关性。PAD-2和PAD-4的mRNA在发炎和非发炎牙龈组织中检测到。抗CCP抗体主要存在于牙周炎患者的龈沟液中。结论炎症牙龈组织中存在PAD-2、PAD-4蛋白和mRNA以及瓜氨酸化蛋白，部分患者龈沟液中可检测到抗CCP抗体。瓜氨酸化蛋白和PAD的组织表达随着炎症的严重程度而增加。抗CCP抗体在龈沟液中的存在几乎仅限于牙周炎患者的一个子集。这些蛋白质在发炎牙龈中的表达增加支持牙周炎以类似于类风湿性关节炎的方式促进炎症负担的观点。
Background and Objective The presence of citrullinated proteins, and peptidylarginine deiminase types -2 (PAD-2) and -4 (PAD-4) in periodontal tissues, determine the presence of anti-cyclic citrullinated protein antibodies (anti-CCP) in gingival crevicular fluid (GCF) and compare the expression of these proteins between inflamed and non-inflamed sites. Material and Methods Tissue sections were stained using antibodies against citrullinated proteins, PAD-2 and PAD-4. RT-PCR was performed to investigate PAD-2 and PAD-4 mRNA in inflamed and non-inflamed gingival tissues. Anti-CCP antibodies in gingival crevicular fluid were detected by ELISA. Results Citrullinated proteins, PAD-2 and PAD-4 were detected in gingiva. There was a correlation between inflammation and expression of these proteins. mRNAs for PAD-2 and PAD-4 were detected in both inflamed and non-inflamed gingival tissues. Antibodies to CCP were found mostly in the GCF of individuals with periodontitis. Conclusion PAD-2 and PAD-4 (protein and mRNA) as well as citrullinated proteins are present in inflamed gingiva, and anti-CCP antibodies can be detected in the GCF of some patients. Tissue expression of citrullinated proteins and PAD increased with the severity of inflammation. The presence of anti-CCP antibodies in GCF was almost exclusive to a subset of patients with periodontitis. Increased expression of these proteins in inflamed gingiva lends support to the notion that periodontal inflammation contributes to the inflammatory burden in a similar way to rheumatoid arthritis.