Nocardia huaxiensis sp. nov., an actinomycete isolated from human skin
Nocardia huaxiensis sp. nov., an actinomycete isolated from human skin
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DOI：
10.1099/ijsem.0.004970
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发表时间：
2021-01-01
影响因子：
2.8
通讯作者：
Ran, Yuping
中科院分区：
文献类型：
--
作者：
Zhuang, Kaiwen;Liu, Ya;Ran, Yuping
从中国四川省成都市华西医院一名患者的皮肤活检标本中分离出一种新型放线菌，命名为菌株WCH-YHL-001(T)。这些细胞是革兰氏阳性、需氧、异养和非运动的。它们形成了一个带有短气生菌丝体的广泛基质，其分支分裂成杆状元件。生长发生在 10-40 摄氏度、pH 5.0-12.0、氯化钠浓度为 0-4.0% (w/v) 的条件下。菌株WCH-YHL-001(T)的主要细胞脂肪酸是C-16:0、C-18:1 omega 9c、C-18(:0) 10-甲基和总特征3。主要呼吸醌是MK-8（H4 omega-环）。主要的极性脂质是磷脂酰乙醇胺、二磷脂酰甘油。磷脂酰肌醇甘露糖苷、未知磷脂和未知糖脂。肽聚糖的诊断二氨基酸是内消旋二氨基庚二酸。全细胞糖模式由阿拉伯糖和葡萄糖组成。基于16S rRNA基因序列的系统发育分析表明，菌株WCH-YHL-001(T)属于诺卡氏菌属。菌株 WCH-YHL-001(T) 和诺卡氏菌属典型菌株之间的平均核苷酸同一性 (ANI) 和计算机 DNA-DNA 杂交 (isDDH) 值低于定义细菌物种所需的临界值（ANI >= 95-96%，isDDH >= 70%）。基因组DNA G+C含量为67.8mol%。系统发育、生理学和化学分类学数据表明菌株WCH-YHL-001(T)代表了诺卡氏菌属的一个新种，其名称为Nocardia huaxiensis sp.。十一月提出了模式菌株WCH-YHL-001(T) (=GDMCC 4.181(T)=JCM 34475(T)=NBRC 114973(T))。
A novel actinomycete, designated as strain WCH-YHL-001(T), was isolated from skin biopsy specimens of a patient at West China Hospital, Chengdu, Sichuan Province, PR China. The cells were Gram-positive, aerobic, heterotrophic and non-motile. They formed an extensive substrate with short aerial mycelia, whose branches fragmented into rod-shaped elements. Growth occurred at 10-40 degrees C, pH 5.0-12.0 and with NaCl concentrations of 0-4.0% (w/v). The major cellular fatty acids of strain WCH-YHL-001(T) were C-16:0, C-18:1 omega 9c, C-18(:0) 10-methyl and summed feature 3. The predominant respiratory quinone was MK-8 (H4 omega-cycl). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol. phosphatidylinositol mannoside, unknown phospholipids and unidentified glycolipids. The diagnostic diamino acid of peptidoglycan was meso-diaminopimelic acid. The whole-cell sugar pattern consisted of arabinose and glucose. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain WCH-YHL-001(T) belonged to the genus Nocardia. The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (isDDH) values between strain WCH-YHL-001(T) and type strains of Nocardia species were lower than the cut-offs (>= 95-96% for ANI and >= 70% for isDDH) required to define a bacterial species. The genomic DNA G+C content was 67.8 mol%. Phylogenetic, physiological and chemotaxonomic data suggested that strain WCH-YHL-001(T) represented a novel species of the genus Nocardia, for which the name Nocardia huaxiensis sp. nov. is proposed, with the type strain WCH-YHL-001(T) (=GDMCC 4.181(T)=JCM 34475(T)=NBRC 114973(T)).