Sequence and annotation of the 314-kb MT325 and the 321-kb FR483 viruses that infect Chlorella Pbi
Sequence and annotation of the 314-kb MT325 and the 321-kb FR483 viruses that infect Chlorella Pbi
复制标题
DOI：
10.1016/j.virol.2006.08.034
复制
发表时间：
2007-02-20
期刊：
影响因子：
3.7
通讯作者：
Van Etten, James L.
中科院分区：
文献类型：
--
作者：
Fitzgerald, Lisa A.;Graves, Michael V.;Van Etten, James L.
病毒 MT325 和 FR483 是藻类DNA病毒科、氯病毒属的成员，感染淡水、单细胞、真核、类小球藻绿藻，小球藻 Pbi。 MT325 的 314,335 bp 基因组和 FR483 的 321,240 bp 基因组是第一个感染小球藻 Pbi 的病毒，其基因组已被测序和注释。此外，这些基因组是迄今为止测序的两个最小的小球藻病毒基因组，MT325有331个假定的蛋白质编码基因和10个tRNA编码基因，FR483有335个假定的蛋白质编码基因和9个tRNA编码基因。蛋白质编码基因几乎均匀分布在两条链上，基因间空间最小。大约 40% 的病毒基因产物与公共数据库中的条目相似，其中一些是首次在病毒中检测到的此类产物。例如，这些独特的基因产物包括 MT325 中的水甘油孔蛋白、FR483 中的钾离子转运蛋白和烷基硫酸酯酶，以及两种病毒中的 dTDP-葡萄糖焦磷酸化酶。将 MT325 和 FR483 蛋白编码基因与原型小球藻病毒 PBCV-I 进行比较表明，大约 82% 的基因存在于所有三种病毒中。 (c) 2006 Elsevier Inc. 保留所有权利。
Viruses MT325 and FR483, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella Pbi. The 314,335-bp genorne of MT325 and the 321,240-bp genome of FR483 are the first viruses that infect Chlorella Pbi to have their genomes sequenced and annotated. Furthermore, these genomes are the two smallest chlorella virus genomes sequenced to date, MT325 has 331 putative protein-encoding and 10 tRNA-encoding genes and FR483 has 335 putative protein-encoding and 9 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands, and intergenic space is minimal. Approximately 40% of the viral gene products resemble entries in public databases, including some that are the first of their kind to be detected in a virus. For example, these unique gene products include an aquaglyceroporin in MT325, a potassium ion transporter protein and an alkyl sulfatase in FR483, and a dTDP-glucose pyrophosphorylase in both viruses. Comparison of MT325 and FR483 protein-encoding genes with the prototype chlorella virus PBCV-I indicates that approximately 82% of the genes are present in all three viruses. (c) 2006 Elsevier Inc. All rights reserved.