Comparative biochemical and RAPD analysis in two varieties of rice (Oryza sativa) under arsenic stress by using various biomarkers
Comparative biochemical and RAPD analysis in two varieties of rice (Oryza sativa) under arsenic stress by using various biomarkers
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DOI：
10.1016/j.jhazmat.2012.03.005
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发表时间：
2012-05-30
影响因子：
13.6
通讯作者：
Gupta, Meetu
中科院分区：
文献类型：
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作者：
Ahmad, Mohd Anwar;Gaur, Rashmi;Gupta, Meetu
多种生物标志物系统已被频繁地用于测量环境污染物（包括重金属）对活生物体的遗传毒性效应。在这项研究中，我们使用的叶片水培生长14天的水稻（Oryza sativa）品种（PB 1和IR 64）的幼苗处理50，150和300亩M的亚砷酸盐（As（III））的24和96小时的时间。减少种子发芽，根冠长度，叶绿素和蛋白质，观察到增加As（III）浓度和持续时间在两个品种，更多的IR 64。抗氧化酶活性和胁迫相关参数在高浓度处理24 h和96 h时均发生了显著变化。在RAPD分析中，有11个引物产生多态性带型，在PB1和IR64中分别产生51条（对照）、79条（处理）和42条（对照）、29条（处理）带型。这些结果表明，基因组模板的稳定性（GTS，在RAPD图谱的变化）显着影响在所有测试的As（III）浓度，与其他参数相比。在两个品种中观察到差异反应，PB1更耐受。因此，DNA多态性的RAPD分析结合其他生化指标可以作为砷污染的生物监测的一个强有力的生态毒理学工具。(C)2012爱思唯尔有限公司版权所有。
Multiple biomarker systems have been frequently used to measure the genotoxic effects of environmental pollutants (including heavy metals) on living organisms. In this study, we used leaves of hydroponically grown 14 days old seedlings of rice (Oryza sativa)varieties (PB1 and IR64) treated with 50,150 and 300 mu M arsenite (As(III)) for 24 and 96 h duration. Reduction in seed germination, root-shoot length, chlorophyll and protein were observed with increasing As(III) concentration and duration in both varieties, being more in IR64. Increase/decrease of antioxidant enzymes and stress related parameters showed much changes at higher concentration for 24 and 96h duration in both varieties. Eleven primers were found in RAPD analysis to produce polymorphic band pattern and produced a total of 51 (control), 79 (treated) and 42 (control) and 29 (treated) bands in PB1 and IR64 varieties, respectively. These results indicated that genomic template stability (GTS, changes in RAPD profile) was significantly affected at all tested As(III) concentration, when compared with other parameters. Differential response was observed in both varieties with PB1 being more tolerant. We concluded that DNA polymorphism detected by RAPD analysis in conjunction with other biochemical parameters could be a powerful eco-toxicological tool in bio-monitoring arsenic pollution. (C) 2012 Elsevier B.V. All rights reserved.