Demystified… recombinant antibodies
Demystified… recombinant antibodies
复制标题
揭秘……重组抗体
DOI：
--
复制
发表时间：
2004
影响因子：
3.4
通讯作者：
J. Greenman
中科院分区：
文献类型：
--
作者：
K. A. Smith;Paul N. Nelson;P. Warren;S. Astley;P. G. Murray;J. Greenman
重组抗体是生物医学研究的重要工具，越来越多地被用作临床诊断/治疗试剂。在这篇文章中，给出了人源化抗体的背景，以及抗体片段产生的细节，例如单链Fv片段。噬菌体抗体片段正迅速流行起来，可以通过简单的建立方法从免疫细胞的文库中产生亲和富集。噬菌体展示法也可用于现有抗体片段的亲和富集，以提供具有更高亲和力的试剂。在这里，噬菌体抗体被揭开了神秘面纱，以提供对这些试剂潜力的更深入的了解，并吸引临床医生和生物医学科学家都考虑在病理和临床环境中的潜在应用。
Recombinant antibodies are important tools for biomedical research and are increasingly being used as clinical diagnostic/therapeutic reagents. In this article, a background to humanised antibodies is given, together with details of the generation of antibody fragments—for example, single chain Fv fragments. Phage antibody fragments are fast becoming popular and can be generated by simple established methods of affinity enrichment from libraries derived from immune cells. Phage display methodology can also be used for the affinity enrichment of existing antibody fragments to provide a reagent with a higher affinity. Here, phage antibodies are demystified to provide a greater understanding of the potential of these reagents and to engage clinicians and biomedical scientists alike to think about potential applications in pathology and clinical settings.
来自人类合成噬菌体展示文库的单链抗体片段可结合血小板反应蛋白并抑制镰状细胞粘附。
影响因子：
20.3
作者：
Watkins,NicholasA;Du,LilyM;Scott,JPaul;Ouwehand,WillemH;Hillery,CherylA
通讯作者：
Hillery,CherylA