Serotonin induces EPSCs preferentially in layer V pyramidal neurons of the frontal cortex in the rat
Serotonin induces EPSCs preferentially in layer V pyramidal neurons of the frontal cortex in the rat
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DOI：
10.1093/cercor/10.10.974
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发表时间：
2000-10-01
期刊：
影响因子：
3.7
通讯作者：
Aghajanian, GK
中科院分区：
文献类型：
--
作者：
Lambe, EK;Goldman-Rakic, PS;Aghajanian, GK
在额叶皮层第 II-VI 层的锥体细胞中检查了血清素 (5-HT) 对谷氨酸释放的影响。细胞内记录电极含有 1% 的生物胞素，因此随后可以使用亲和素-生物素过氧化物酶方法对神经元进行可视化。额叶皮层第五层的锥体细胞表现出 5-HT 诱导的“自发”（非电诱发）兴奋性突触后电流 (EPSC) 频率和幅度的最大增加。 II/III 层中的一小部分神经元显示 EPSC 频率增加，而 VI 层中的细胞显示 EPSC 频率或幅度没有显着变化。对 5-HT 的生理反应反映了 V 层中 5-HT2A 受体的高密度，以及额叶皮质中丘脑投射的模式。 EPSC 在 V 层神经元中的特异性诱导表明 5-HT 优先调节额叶皮层的输出神经元。
The effect of serotonin (5-HT) on the release of glutamate was examined in pyramidal cells in layers II-VI of the frontal cortex. The intracellular recording electrode contained 1% biocytin so the neurons could later be visualized with an avidin-biotin peroxidase method. Pyramidal cells in layer V of the frontal cortex showed the greatest 5-HT-induced increase in both the frequency and amplitude of 'spontaneous' (non-electrically evoked) excitatory post-synaptic currents (EPSCs). A small proportion of neurons in layer II/III showed an increase in EPSC frequency, whereas cells in layer VI showed no significant change in either EPSC frequency or amplitude. The physiological response to 5-HT mirrors the high density of 5-HT2A receptors in layer V, as well as the pattern of thalamic projections in frontal cortex. The specific induction of EPSCs in layer V neurons suggests that 5-HT preferentially modulates the output neurons of the frontal cortex.