HUMAN LANGERHANS CELLS EXPRESS E-CADHERIN
HUMAN LANGERHANS CELLS EXPRESS E-CADHERIN
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DOI：
10.1111/1523-1747.ep12612830
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发表时间：
1995-02-01
影响因子：
6.5
通讯作者：
UDEY, MC
中科院分区：
文献类型：
--
作者：
BLAUVELT, A;KATZ, SI;UDEY, MC
小鼠郎格汉斯细胞(LC)体外合成并表达E-钙粘附素，E-钙粘附素是一种钙离子依赖的嗜同型细胞黏附分子，介导LC与角质形成细胞(KC)的结合。在体内，LC表达E-钙粘附素可能通过LC-KC的黏附促进LC在表皮内的定位和持久性。此外，LC E-钙粘附素表达或亲和力的改变可能是LC暴露于抗原后从表皮渗出的一个重要因素。本研究的目的是确定人类LC是否也表达E-钙粘附素。取正常志愿者的负压吸泡顶板，在1 nM Ca++存在下，用有限胰酶消化法制备表皮细胞悬液。将E-钙粘素和CD1a或人类白细胞抗原DR的抗体与EC孵育，用双色分析流式细胞仪或免疫荧光显微镜观察。大多数新鲜制备的人LC(82.9%+/-7.4%[Mean+/-SD]，范围67-89%，n=7)表达E-钙粘蛋白，大多数KC也表达E-钙粘蛋白。LC和KC表达的E-钙粘附素的量(由平均荧光强度确定)相似。胰酶/EDTA处理新制备的EC可抑制LC和KC对E-钙粘蛋白的表达，但在有钙离子存在的情况下，胰酶不能降解E-钙粘附素。LC在培养3d后表达较低水平的E-钙粘附素。因此，人LC和小鼠LC一样，表达亲水性黏附分子E-钙粘附素，这可能在哺乳动物表皮中建立和维持LC与KC之间的相互作用中起重要作用。
Murine Langerhans cells (LC) synthesize and express E-cadherin, a Ca++-dependent homophilic cell adhesion molecule that mediates LC-keratinocyte (KC) binding in vitro. In vivo, E-cadherin expression by LC may promote localization and persistence of LC within the epidermis through LC-KC adhesion. In addition, changes in LC E-cadherin expression or affinity may be an important factor in the egress of LC from the epidermis after exposure to antigen. The aim of the present study was to determine if human LC also express E-cadherin. Suction blister roofs were obtained from normal volunteers and epidermal cell (EC) suspensions were prepared by limited trypsinization in the presence of 1 nM Ca++. EC were then incubated with antibodies to E-cadherin and CD1a or HLA-DR, and examined by two-color analytical flow cytometry or immunofluorescence microscopy. Most (82.9% +/- 7.4% [mean +/- SD], range 67-89%, n = 7) freshly prepared human LC expressed E-cadherin, as did the majority of KC. The amount of E-cadherin (as determined by mean fluorescence intensity) expressed by LC and KC was similar. Trypsin/EDTA treatment of freshly prepared EC abrogated expression of E-cadherin by LC and KC, whereas E-cadherin was not degraded by trypsin in the presence of Ca++. LC expressed lower levels of E-cadherin after 3 d in culture. Thus, human LC, like murine LC, express the homophilic adhesion molecule E-cadherin, which may be important in establishing and maintaining interactions between LC and KC in mammalian epidermis.