Fiber optic microendoscopy for preclinical study of bacterial infection dynamics.
Fiber optic microendoscopy for preclinical study of bacterial infection dynamics.
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DOI：
10.1364/boe.2.001121
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发表时间：
2011-04-07
影响因子：
3.4
通讯作者：
Maitland KC
中科院分区：
文献类型：
--
作者：
Mufti N;Kong Y;Cirillo JD;Maitland KC
我们探索使用纤维光学显微内窥镜成像和量化细菌感染的皮肤和肺部动物模型。接触探针光纤束荧光显微内窥镜具有4 µm分辨率、750 µm视场和1 mm外径。从接种物分别降至104和107个菌落形成单位时，原位检测到荧光牛分枝杆菌卡介苗（BCG）的皮下和气管内感染。
We explore the use of fiber optic microendoscopy to image and quantify bacterial infection in the skin and lungs using an animal model. The contact probe fiber bundle fluorescence microendoscope has a 4 µm resolution, a 750 µm field of view, and a 1 mm outer diameter. Subcutaneous and intra-tracheal infections of fluorescent Mycobacterium bovis Bacillus Calmette-Guérin (BCG) bacteria were detected in situ from inocula down to 104 and 107 colony forming units, respectively.