A novel deltapartitivirus from red clover
A novel deltapartitivirus from red clover
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DOI：
10.1007/s00705-022-05372-3
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发表时间：
2022-03-04
影响因子：
2.7
通讯作者：
Suzuki，Nobuhiro
中科院分区：
文献类型：
--
作者：
Telengech，Paul;Shahi，Sabitree;Suzuki，Nobuhiro
该病毒家族有5个属，其中包括三角洲病毒属。我们在这里报告了一种来自红三叶草的三角病毒的完整基因组序列，称为“红三叶草隐病毒3”（RCCV3）。RCCV3具有双链RNA基因组。dsRNA1和dsRNA2的长度分别为1580和1589个核苷酸（nt），预计分别编码RNA定向RNA聚合酶（RdRP）和衣壳蛋白（CP）。RCCV3的RdRP序列与先前报道的苜蓿三角洲病毒1型（MsDPV1）的RdRP序列同源性最高（76.5%），而RCCV3的CP序列与MsDPV1的CP序列同源性为50%。基于RdRP和cp的系统发育树将RCCV3归入三角洲病毒的一个分支。序列分析和系统发育分析表明，RCCV3是deltapartitivirus属的一个新种。在3个红三叶草品种中均检测到RCCV3。
The familyPartitiviridaehas five genera, among which is the genusDeltapartitivirus. We report here the complete genome sequence of a deltapartitivirus from red clover, termed “red clover cryptic virus 3” (RCCV3). RCCV3 has a bisegmented double-stranded (ds) RNA genome. dsRNA1 and dsRNA2 are 1580 and 1589 nucleotides (nt) in length and are predicted to encode an RNA-directed RNA polymerase (RdRP) and a capsid protein (CP), respectively. The RCCV3 RdRP shares the highest sequence identity with the RdRP of a previously reported deltapartitivirus, Medicago sativa deltapartitivirus 1 (MsDPV1) (76.5%), while the RCCV3 CP shows 50% sequence identity to the CP of MsDPV1. RdRP- and CP-based phylogenetic trees place RCCV3 into a clade of deltapartitiviruses. The sequence and phylogenetic analyses clearly indicate that RCCV3 represents a new species in the genusDeltapartitivirus. RCCV3 was detectable in all three tested cultivars of red clover.