Cathepsin B/L-, elastase-, tryptase-, trypsin- and dipeptidyl peptidase IV-like activities in gingival crevicular fluid: correlation with clinical parameters in untreated chronic periodontitis patients.
Cathepsin B/L-, elastase-, tryptase-, trypsin- and dipeptidyl peptidase IV-like activities in gingival crevicular fluid: correlation with clinical parameters in untreated chronic periodontitis patients.
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龈沟液中的组织蛋白酶 B/L、弹性蛋白酶、类胰蛋白酶、胰蛋白酶和二肽基肽酶 IV 样活性：与未经治疗的慢性牙周炎患者的临床参数的相关性。
DOI：
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发表时间：
1992
影响因子：
3.5
通讯作者：
S. Cox
中科院分区：
文献类型：
--
作者：
B. Eley;S. Cox
对20名未经治疗的慢性牙周炎患者进行了全面的牙周检查，包括探诊深度（PD）、临床附着丧失（CAL）、牙龈指数（GI）、出血指数（BI）和菌斑指数（Pl.I.）的测量。在第二次就诊时，从每颗牙齿最深的可触及探测部位用滤纸条收集龈沟液（GCF）。测定 GCF 体积并将样品洗脱到缓冲液中。使用 7-氨基-4-三氟甲基香豆素 (AFC) 的肽基衍生物测定所得洗脱液中的蛋白酶活性。组织蛋白酶 B/L 样活性用 Bz-Val-Lys-Lys-Arg-AFC 测定，弹性蛋白酶样活性用 MeOSuc-Ala-Ala-Pro-Val-AFC 测定，类胰蛋白酶样活性用 Z-Ala-Ala-Lys-AFC 测定，胰蛋白酶样活性用 Z-Gly-Gly-Arg-AFC 测定，二肽基肽酶 (DPP) IV 样活性与 Ala-Pro-AFC 一起。线性回归分析中总酶活性和酶浓度均与所有临床参数呈正相关。在使用患者平均值的患者级别和使用个体患者或汇总患者数据的站点级别上都是如此。大多数相关性具有统计显着性，尽管总酶活性的比例大于浓度。对于总活性，不同酶和参数的相关性通常遵循以下顺序：组织蛋白酶 B/L-大于弹性蛋白酶-大于 DPP IV-大于胰蛋白酶-大于类胰蛋白酶活性，PD 大于 CAL 大于 GI 大于 BI 大于 PI.I。在这项横断面研究中，总酶活性作为临床参数的预测因子具有良好的诊断特异性和敏感性，表明 GCF 蛋白酶可能提供有关牙周状况的有用信息。
20 untreated chronic periodontitis patients were given a full periodontal examination, including measurements of probing depth (PD), clinical attachment loss (CAL), gingival index (GI), bleeding index (BI) and plaque index (Pl.I.). At a second visit, gingival crevicular fluid (GCF) was collected on filter paper strips from the deepest accessible probing site of each tooth. GCF volumes were determined and the samples eluted into buffer. Protease activities in the resulting eluates were assayed with peptidyl derivatives of 7-amino-4-trifluoromethyl coumarin (AFC). Cathepsin B/L-like activity was determined with Bz-Val-Lys-Lys-Arg-AFC, elastase-like activity with MeOSuc-Ala-Ala-Pro-Val-AFC, tryptase-like activity with Z-Ala-Ala-Lys-AFC, trypsin-like activity with Z-Gly-Gly-Arg-AFC and dipeptidyl peptidase (DPP) IV-like activity with Ala-Pro-AFC. Total enzyme activities and enzyme concentrations both correlated positively with all clinical parameters in linear regression analysis. This was true on both a patient level, using mean patient values, and a site level, using either individual patient or pooled patient data. Most of these correlations were statistically significant, although the proportion was greater for total enzyme activity than concentration. With total activities, correlations with different enzymes and parameters generally followed the order: cathepsin B/L-greater than elastase- greater than DPP IV- greater than trypsin- greater than tryptase-like activity and PD greater than CAL greater than GI greater than BI greater than Pl.I respectively. Total enzyme activities had good diagnostic specificity and sensitivity as predictors of clinical parameters in this cross-sectional study, suggesting that GCF proteases might provide useful information on the periodontal condition.
哺乳动物胶原酶的调节。
DOI：
10.1016/s0174-173x(84)80015-1
发表时间：
1984
期刊：
Collagen and related research
影响因子：
--
作者：
HarrisJr,ED;Welgus,HG;Krane,SM
通讯作者：
Krane,SM
人肥大细胞类胰蛋白酶激活潜在的类风湿滑膜胶原酶。
DOI：
--
发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Gruber,BL;Schwartz,LB;Ramamurthy,NS;Irani,AM;Marchese,MJ
通讯作者：
Marchese,MJ