Floating culture promotes the maintenance of hematopoietic stem cells
Floating culture promotes the maintenance of hematopoietic stem cells
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DOI：
10.1016/j.febslet.2007.08.057
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发表时间：
2007-10-02
期刊：
影响因子：
3.5
通讯作者：
Kobayashi, Masao
中科院分区：
文献类型：
--
作者：
Kajiume, Teruyuki;Yuge, Louis;Kobayashi, Masao
在这项研究中，我们研究了造血干细胞（HSC）维持的频率上的培养基的比重的影响。我们使用了一种新开发的高比重介质。分离培养骨髓细胞，测定HSC活性。在高比重的培养基中，造血祖/干细胞的数量显著增加。在高比重培养基中，细胞不沉淀，HSC的维持增加，并且伴随着β-连环蛋白的积累。这种维持HSC群体的新技术为再生医学研究提供了重要的新工具。(c)2007年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
In this study we examined the effect of the specific gravity of culture medium on the frequency of hematopoietic stem cell (HSC) maintenance. We used a newly developed high-specific-gravity media. Bone marrow cells were isolated and cultured, and HSC activity was evaluated. The number of hematopoietic progenitor/stem cells was markedly higher in the medium with high specific gravity. In high-specific-gravity media, cells did not precipitate, maintenance of HSCs was increased, and there was a concomitant accumulation of beta-catenin. This novel technique for maintaining HSC populations provides an important new tool for studies in regenerative medicine. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.